Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
J Insect Sci. 2011;11:10. doi: 10.1673/031.011.0110.
Eight isoline colonies of Anopheles peditaeniatus Leicester (Diptera: Culicidae) were established from wild-caught females collected from buffalo-baited traps at 8 localities in Thailand. They showed 2 types of X (X(2), X(3)) and 4 types of Y (Y(2), Y(3), Y(4), Y(5)) chromosomes based on the number and amount of major block(s) of heterochromatin present in the heterochromatic arm, and were tentatively designated as Forms B (X(2), X(3), Y(2)), C (X(3), Y(3)), D (X(3), Y(4)) and E (X(2), X(3), Y(5)). Form B was found in Nan, Ratchaburi, and Chumphon provinces; Form C was obtained in Chon Buri province; Form D was recovered in Kamphaeng Phet province; and Form E was acquired in Chiang Mai, Udon Thani, and Ubon Ratchathani provinces. Crossing studies among the 8 isoline colonies, which were representative of 4 karyotypic forms of An. peditaeniatus, revealed genetic compatibility in providing viable progenies and synaptic salivary gland polytene chromosomes through F(2)-generations, thus suggesting the conspecific nature of these karyotypic forms. These results were supported by the very low intraspecific sequence variations (0.0 - 1.1%) of the nucleotide sequences in ribosomal DNA (ITS2) and mitochondrial DNA (COI and COII) of the 4 forms.
从泰国 8 个地点的水牛诱捕陷阱中捕获的野生雌蚊建立了 8 个莱斯特氏按蚊（双翅目：蚊科）的等线种群。根据异染色质臂中存在的主要异染色质块的数量和量，它们显示出 2 种 X（X(2)，X(3)）和 4 种 Y（Y(2)，Y(3)，Y(4)，Y(5)）染色体，并被暂定命名为 B 型（X(2)，X(3)，Y(2)）、C 型（X(3)，Y(3)）、D 型（X(3)，Y(4)）和 E 型（X(2)，X(3)，Y(5)）。B 型在那空、叻丕和春蓬省发现；C 型在春武里府获得；D 型在甘烹碧府回收；E 型在清迈、乌汶叻差他尼和乌隆他尼府获得。代表按蚊 peditaeniatus 4 种核型形式的 8 个等线种群的杂交研究表明，通过 F(2)-代提供了有活力的后代和突触唾液腺多线染色体的遗传相容性，因此表明这些核型形式具有同物异性。这些结果得到了 4 种形式的核糖体 DNA（ITS2）和线粒体 DNA（COI 和 COII）的核苷酸序列的种内序列变异（0.0-1.1%）非常低的支持。
