Taai K, Baimai V, Thongsahuan S, Saeung A, Otsuka Y, Srisuka W, Sriwichai P, Somboon P, Jariyapan N, Choochote W
Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
Trop Biomed. 2013 Jun;30(2):238-49.
Sixteen isoline colonies of Anopheles paraliae were established from wild-caught females collected from cow-baited traps at 4 locations in Thailand. They showed 3 types of X (X1, X2, X3) and 5 types of Y (Y1, Y2, Y3, Y4, Y5) chromosomes based on the number and amount of major block(s) of heterochromatin present in the heterochromatic arm, and were designated as Forms A (X3, Y1), B (X1, X2, X3, Y2), C (X3, Y3), D (X1, X2, X3, Y4) and E (X3, Y5). Form A was found in Songkhla Province, Form B was obtained in Ratchaburi, Nakhon Si Thammarat and Songkhla Provinces, Form C was acquired in Chanthaburi Province, Form D was recovered in Ratchaburi and Songkhla Provinces, and Form E was encountered in Ratchaburi Province. Hybridization experiments among the 7 isoline colonies, which represented the 5 karyotypic forms of An. paraliae, revealed genetic compatibility in providing viable progenies and synaptic salivary gland polytene chromosomes through F2-generations, and thus suggest the conspecific nature of these karyotypic forms. These results were supported by the very low intraspecific sequence divergence (mean genetic distance = 0.000-0.002) of the nucleotide sequences in ribosomal DNA (ITS2) and mitochondrial DNA (COI and COII) of the 5 forms.
从泰国4个地点的牛诱饵诱捕器中捕获的野生雌性按蚊建立了16个同线菌落。根据异染色质臂中存在的异染色质主要区段的数量和大小，它们显示出3种X染色体类型（X1、X2、X3）和5种Y染色体类型（Y1、Y2、Y3、Y4、Y5），并被指定为A（X3，Y1）、B（X1、X2、X3，Y2）、C（X3，Y3）、D（X1、X2、X3，Y4）和E（X3，Y5）型。在宋卡府发现了A型，在叻丕府、洛坤府和宋卡府获得了B型，在尖竹汶府获得了C型，在叻丕府和宋卡府发现了D型，在叻丕府发现了E型。代表按蚊5种核型形式的7个同线菌落之间的杂交实验表明，通过F2代可以产生有活力的后代并形成联会的唾液腺多线染色体，从而表明这些核型形式属于同种。核糖体DNA（ITS2）和线粒体DNA（COI和COII）的核苷酸序列在这5种形式中的种内序列差异非常低（平均遗传距离 = 0.000 - 0.002），支持了这些结果。
