Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
Mem Inst Oswaldo Cruz. 2009 Jul;104(4):558-66. doi: 10.1590/s0074-02762009000400004.
Seventy-one isolines of Anopheles campestris-like were established from wild-caught females collected from human-biting and animal-biting traps at 12 locations in Thailand. All isolines had an average branch summation of seta 2-VI pupal skins ranging from 20.3-30.0 branches, which is in the range of An. campestris (17-58 branches). They showed three different karyotypes based on the amount of extra heterochromatin in the sex chromosomes, namely Forms B (X2, Y2), E (X1, X2, X3, Y5) and a new karyotypic Form F (X2, X3, Y6). Form B has been found only in Chaing Mai and Kamphaeng Phet populations, while Forms E and F are widely distributed throughout the species range. Genetic crosses between the 12 isolines, which were arbitrarily selected as representatives of An. campestris-like Forms B, E and F, revealed genetic compatibility that provided viable progeny through F2 generations, suggesting a conspecific nature of these karyotypic forms. These results are supported by the very low intraspecies variation (genetic distance < 0.005) of ITS2, COI and COII from genomic DNA of the three karyotypic forms.
从泰国 12 个地点的人蚊和动物诱捕器中捕获的雌性野生蚊子中建立了 71 条类似按蚊的隔离种群。所有的隔离种群的 2-VI 蛹皮的平均分支总和范围为 20.3-30.0 个分支，这在按蚊属（17-58 个分支）范围内。它们根据性染色体上额外异染色质的数量显示出三种不同的核型，即 B 型（X2，Y2）、E 型（X1，X2，X3，Y5）和一种新的核型 F 型（X2，X3，Y6）。B 型仅在清迈和甘烹碧府种群中发现，而 E 型和 F 型则广泛分布于该物种的分布范围内。在 12 个隔离种群之间进行的遗传杂交，这些隔离种群被任意选择为代表 B、E 和 F 型的类似按蚊形式，结果显示出遗传兼容性，通过 F2 代提供了有活力的后代，表明这些核型形式具有同一种属的性质。这些结果得到了来自三个核型形式的基因组 DNA 的 ITS2、COI 和 COII 的非常低的种内变异（遗传距离<0.005）的支持。
