Willmitzer L, Schmalenbach W, Schell J
Nucleic Acids Res. 1981 Oct 10;9(19):4801-12. doi: 10.1093/nar/9.19.4801.
Highly purified and physiologically active nuclei were isolated from four different octopine and nopaline crown gall lines. These nuclei exhibited a high endogenous RNA synthesizing activity involving all three RNA-polymerases I, II and III. Isolated nuclei were shown by Southern blotting to synthesize T-DNA specific RNA. This synthesis was shown to be sensitive to actinomycin D and therefore to be DNA-dependent. The transcription of the T-DNA was also inhibited for more than 90% by low concentrations of alpha-amanitin (0.7 micrograms/ml) indicating that the T-DNA, although from bacterial origin, is transcribed by the host RNA polymerase II.
从四种不同的章鱼碱型和胭脂碱型冠瘿瘤系中分离出了高度纯化且具有生理活性的细胞核。这些细胞核表现出较高的内源性RNA合成活性，涉及所有三种RNA聚合酶I、II和III。通过Southern印迹法显示，分离出的细胞核能合成T-DNA特异性RNA。这种合成对放线菌素D敏感，因此是依赖DNA的。低浓度的α-鹅膏蕈碱（0.7微克/毫升）也能抑制T-DNA的转录达90%以上，这表明T-DNA虽然起源于细菌，但却是由宿主RNA聚合酶II转录的。
