Department of Biochemistry and Nutrition, Central Food Technological Research Institute, Council of Scientific and Industrial Research, Mysore, India.
Br J Nutr. 2011 Aug;106(4):530-9. doi: 10.1017/S0007114511000663. Epub 2011 Apr 27.
Vegetable oils containing α-linolenic acid (ALA; 18 : 3n-3) have been shown to modulate the functions of immunocompetent cells. The aim of the present study was to understand the modulatory effect of ALA-rich garden cress (Lepidium sativum L.) seed oil (GCO) on lipid composition, spleen lymphocyte (SL) proliferation and inflammatory mediator production by peritoneal macrophages (PMΦ) in rats. Female Wistar rats were fed diets containing either GCO (2·5, 5·0 and 10 %, w/w) or sunflower oil (SFO, 10 % w/w) for 8 weeks. Ex vivo proliferation of SL was measured by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. IL-2 and TNF-α in SL and PMΦ were analysed by ELISA. Inflammatory mediators such as NO, leukotriene B(4) (LTB(4)) and H(2)O(2) were measured in mitogen-activated PMΦ. GCO significantly increased the levels of ALA, EPA and DHA, but reduced linoleic acid and arachidonic acid in SL and PMΦ lipids. GCO (10 %) significantly decreased the concanavalin A (Con-A)- and phytohaemagglutinin-mediated proliferation of SL by 54 and 38 %, respectively, in comparison with SFO. A marginal decrease in IL-2 and TNF-α was observed in Con-A-stimulated SL and lipopolysaccharide-stimulated PMΦ. LTB(4) levels in Ca ionophore-stimulated PMΦ were reduced by 40 % in GCO-fed rats. NO release in response to various stimuli was significantly decreased in PMΦ of GCO-fed rats. The present study is the first report on the modulatory efficacy of GCO on immunomediators in rats. GCO modulated inflammatory mediators such as NO and LTB(4), and thus may play a role in alleviating inflammatory conditions favourably.
富含α-亚麻酸（ALA；18：3n-3）的植物油已被证明可调节免疫活性细胞的功能。本研究旨在了解富含ALA 的花园水芹（Lepidium sativum L.）籽油（GCO）对大鼠脂质组成、脾淋巴细胞（SL）增殖和腹腔巨噬细胞（PMΦ）炎症介质产生的调节作用。雌性 Wistar 大鼠连续 8 周分别喂食含有 GCO（2.5、5.0 和 10%，w/w）或葵花籽油（SFO，10%w/w）的饮食。通过 3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐测定法测量 SL 的体外增殖。通过 ELISA 分析 SL 和 PMΦ 中的 IL-2 和 TNF-α。在有丝分裂原激活的 PMΦ 中测量炎症介质如 NO、白三烯 B（4）（LTB（4））和 H（2）O（2）。GCO 可显著增加 ALA、EPA 和 DHA 的水平，但降低 SL 和 PMΦ 脂质中的亚油酸和花生四烯酸。与 SFO 相比，GCO（10%）可使 Con-A 和植物血凝素介导的 SL 增殖分别降低 54%和 38%。在 Con-A 刺激的 SL 和脂多糖刺激的 PMΦ 中观察到 IL-2 和 TNF-α 的轻微下降。GCO 喂养的大鼠中 Ca2+载体刺激的 PMΦ 中 LTB（4）水平降低 40%。GCO 喂养的大鼠对各种刺激的 NO 释放明显降低。本研究首次报道了 GCO 对大鼠免疫调节剂的调节作用。GCO 调节了 NO 和 LTB（4）等炎症介质，因此可能在缓解炎症条件方面发挥作用。
