Horn Patricia, Nausch Henrik, Baars Susanne, Schmidtke Jörg, Schmidt Kerstin, Schneider Anja, Leister Dario, Broer Inge
University of Rostock, Faculty of Agricultural and Environmental Sciences, Department of Agrobiotechnology and Risk Assessment for Bio- und Gene Technology, Justus-von-Liebig Weg 8, 18059 Rostock, Germany.
BioMath GmbH, Friedrich-Barnewitz-Str. 8, 18119, Rostock-Warnemünde, Germany.
Biotechnol Rep (Amst). 2017 Nov 3;16:26-31. doi: 10.1016/j.btre.2017.11.001. eCollection 2017 Dec.
As already demonstrated in greenhouse trials, outcrossing of transgenic plants can be drastically reduced via transgene integration into the plastid. We verified this result in the field with , for which the highest paternal leakage has been observed. The variety white 115 (W115) served as recipient and Pink Wave (PW) and the transplastomic variant PW T16, encoding the A reporter gene, as pollen donor. While manual pollination in the greenhouse led to over 90% hybrids for both crossings, the transgenic donor resulted only in 2% hybrids in the field. Nevertheless paternal leakage was detected in one case which proves that paternal inheritance of plastid-located transgenes is possible under artificial conditions. In the greenhouse, paternal leakage occurred in a frequency comparable to published results. As expected natural pollination reduced the hybrid formation in the field from 90 to 7.6% and the transgenic donor did not result in any hybrid.
正如在温室试验中已经证明的那样，通过将转基因整合到质体中，可以大幅减少转基因植物的异花授粉。我们在田间对[具体作物]验证了这一结果，在该作物中已观察到最高的父本泄漏率。白色115（W115）品种用作受体，粉色波浪（PW）和编码A报告基因的转质体变体PW T16用作花粉供体。虽然温室中的人工授粉在两次杂交中都产生了超过90%的杂种，但转基因供体在田间仅产生了2%的杂种。然而，在一个案例中检测到了父本泄漏，这证明了在人工条件下质体定位转基因的父本遗传是可能的。在温室中，父本泄漏的频率与已发表的结果相当。正如预期的那样，自然授粉使田间的杂种形成从90%降至7.6%，转基因供体没有产生任何杂种。
