Democritus University of Thrace, Faculty of Agricultural Development, Laboratory of Microbiology, Biotechnology and Hygiene, 193 Pandazidou Str., GR68200 Orestiada, Greece.
Anaerobe. 2011 Dec;17(6):399-402. doi: 10.1016/j.anaerobe.2011.03.024. Epub 2011 Apr 16.
Aromatic plants have been used widely to extend the shelf life of foods but at the same time research is undergoes for their properties as antibacterial agents in clinical use. Although there are promising results for the antimicrobial properties of various essential oils against environmental or food-isolated strains of Staphylococcus aureus, limited work has been done concerning these properties against clinical isolates of this pathogen. S. aureus is responsible for an increase number of nosocomial infections and at the same time exhibits increased resistance to synthetic agents. In this study, essential oils from eight aromatic plants common in Greece were isolated by hydrodistillation, analyzed by gas chromatography (GC) and GC/mass spectrometry (GC/MS) for their chemical components and tested for their antimicrobial activities against 24 clinical isolates of S. aureus. The methods used were disk diffusion and broth dilution in order to determine the Minimum Inhibitory Concentration (MIC). Our results showed that essential oils from Origanum vulgare and Origanum dictamnus were active against S. aureus when tested by disk diffusion, but exhibited increased MIC values (>256 mg/L) with the dilution method. In contrast, the reference strain NCTC 6571 showed to be extremely sensitive in most of the oils tested (MICs 0.25-32.0 mg/L) and resistant only to the essential oil from Ocimum basilicum. Therefore, there is no evidence of a potential clinical use for those essential oils and further research is needed in order to determine if they could substitute efficiently synthetic antibiotics or, perhaps be used in combination.
芳香植物被广泛用于延长食品的保质期，但同时也在研究它们作为临床抗菌剂的特性。虽然各种精油对环境或食物中分离出的金黄色葡萄球菌菌株具有抗菌特性的研究结果很有前景，但针对这种病原体的临床分离株的相关研究还很有限。金黄色葡萄球菌是导致医院感染增加的原因，同时其对合成药物的耐药性也在增加。在这项研究中，通过水蒸气蒸馏法从希腊常见的 8 种芳香植物中提取精油，用气相色谱（GC）和气相色谱/质谱（GC/MS）进行化学成分分析，并测试其对 24 株临床分离金黄色葡萄球菌的抗菌活性。采用纸片扩散法和肉汤稀释法来测定最小抑菌浓度（MIC）。我们的研究结果表明，通过纸片扩散法测试时，牛至和荆芥精油对金黄色葡萄球菌具有活性，但稀释法的 MIC 值较高（>256mg/L）。相比之下，参考菌株 NCTC 6571 在大多数测试的精油中表现出极高的敏感性（MIC 值为 0.25-32.0mg/L），仅对罗勒精油表现出耐药性。因此，这些精油没有潜在的临床应用证据，需要进一步研究以确定它们是否可以有效地替代合成抗生素，或者是否可以联合使用。
