Department of Microbiology, Research Center of Barij Essence, 87135-1178 Kashan, Iran.
Phytomedicine. 2010 Jun;17(7):548-50. doi: 10.1016/j.phymed.2009.11.004. Epub 2010 Feb 18.
Staphylococcus aureus is recognized as one of the major cause of infections in communities and hospitals. In this study, the chemical composition and antistaphylococcal activity of Zataria multiflora Boiss essential oil were evaluated, along with its synergistic activity with vancomycin. The essential oil was obtained by hydrodistillation from aerial parts of the plant and analyzed using GC and GC-MS. Thymol (38.7%), carvacrol (15.3%) and rho-cymene (10.2%) were the main components of the oil. Antibacterial activity of the oil was evaluated by microbroth dilution assay against clinical isolates of methicillin -resistant (MR) and methicillin-sensitive (MS) strains of S. aureus. The oil showed high antibacterial activity against MRSA and MSSA with minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) values in the range of 0.25-1 and 0.5-2microl/ml, respectively. The synergistic action was evaluated by microtiter assay. The essential oil exhibited synergism with vancomycin and displayed the ability to enhance the activity of vancomycin. This study showed that Z. multiflora oil has significant antimicrobial activity against clinical isolates of S. aureus, especially MRSA, and might be useful in controlling MRSA infections.
金黄色葡萄球菌被认为是社区和医院感染的主要原因之一。在这项研究中，评估了藏茴香精油的化学成分和抗葡萄球菌活性，以及它与万古霉素的协同活性。精油通过水蒸馏从植物的地上部分获得，并使用 GC 和 GC-MS 进行分析。百里酚（38.7%）、香芹酚（15.3%）和对伞花烃（10.2%）是油的主要成分。通过微量肉汤稀释法评估油对耐甲氧西林金黄色葡萄球菌（MRSA）和甲氧西林敏感金黄色葡萄球菌（MSSA）的临床分离株的抗菌活性。该油对 MRSA 和 MSSA 具有高抗菌活性，最小抑菌浓度（MIC）和最小杀菌浓度（MBC）值分别在 0.25-1 和 0.5-2microl/ml 范围内。通过微量滴定法评估协同作用。精油与万古霉素表现出协同作用，并显示出增强万古霉素活性的能力。这项研究表明，藏茴香精油对金黄色葡萄球菌的临床分离株具有显著的抗菌活性，特别是对耐甲氧西林金黄色葡萄球菌，可能有助于控制耐甲氧西林金黄色葡萄球菌感染。
