在非变性条件下从染色质中分离H1组蛋白的一步分级分离法。
One-step fractionation method for isolating H1 histones from chromatin under nondenaturing conditions.
作者信息
Garcia-Ramirez M, Leuba S H, Ausio J
机构信息
Department of Biochemistry and Biophysics, Oregon State University, Corvallis 97331-6503.
出版信息
Protein Expr Purif. 1990 Sep;1(1):40-4. doi: 10.1016/1046-5928(90)90043-x.
PMID:2152183
Abstract
By combining conventional methods of chromatin preparation with ion-exchange chromatography in CM-Sephadex C-25, we have been able to isolate and fractionate histone H1 variants from different sources. This method of fractionation is very simple and allows one to obtain, very rapidly, large amounts of these histones in a native nondenatured conformation.
摘要
通过将传统的染色体制备方法与CM-葡聚糖凝胶C-25中的离子交换色谱相结合，我们已经能够从不同来源分离和分级分离组蛋白H1变体。这种分级分离方法非常简单，能够让人非常快速地获得大量处于天然未变性构象的这些组蛋白。
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