National Institute of Animal Science, Rural Development Administration, Suwon, Korea.
J Agric Food Chem. 2011 May 25;59(10):5657-64. doi: 10.1021/jf200217j. Epub 2011 Apr 27.
In previous proteomic studies, heat shock protein β 1 (HSPB1) was detected as a candidate protein related to meat quality in cattle. This study sought to determine if its gene expression was associated with intramuscular fat content in the longissimus thoracis muscle of Korean cattle (Hanwoo). Tissue from two groups of 10 steers each, low-marbling (mean intramuscular fat content, 7.4 ± 1.5%) and high-marbling (23.5 ± 2.8%), were used for immunoblotting, real-time PCR, and statistical analyses. HSPB1 expression in both mRNA and protein was shown to be negatively related to intramuscular fat content (P < 0.05). Pathway analysis found two genes, TNF receptor superfamily member 6 (FAS) and angiotensinogen (AGT), that were regulators of the HSPB1 gene. The expression of the two genes showed a negative correlation with intramuscular fat content (P < 0.05). These results suggest that HSPB1, FAS, and AGT may be good candidate genes associated with intramuscular fat content in the longissimus muscle of Korean cattle.
在之前的蛋白质组学研究中，热休克蛋白 β 1（HSPB1）被检测为与牛的肉质相关的候选蛋白。本研究旨在确定其基因表达是否与韩牛（韩牛）胸最长肌的肌内脂肪含量有关。每组使用 10 头阉牛的组织，低大理石纹（平均肌内脂肪含量为 7.4±1.5%）和高大理石纹（23.5±2.8%），用于免疫印迹、实时 PCR 和统计分析。结果表明 HSPB1 的 mRNA 和蛋白表达均与肌内脂肪含量呈负相关（P<0.05）。通路分析发现两个基因，肿瘤坏死因子受体超家族成员 6（FAS）和血管紧张素原（AGT），是 HSPB1 基因的调节剂。这两个基因的表达与肌内脂肪含量呈负相关（P<0.05）。这些结果表明 HSPB1、FAS 和 AGT 可能是与韩牛胸最长肌肌内脂肪含量相关的候选基因。
