Suh Jung-Keun, Lee Jae-Sung, Kong Hongsik, Lee Yoonseok, Lee Hong-Gu
Department of Animal Science and Technology, Sanghuh College of Life Sciences, Konkuk University, Seoul, 05029, Republic of Korea.
Team of An Educational Program for Specialists in Global Animal Science, Brain Korea 21 Plus Project, Sanghuh College of Life Sciences, Konkuk University, Seoul, 05029, Republic of Korea.
Arch Anim Breed. 2020 Nov 17;63(2):417-422. doi: 10.5194/aab-63-417-2020. eCollection 2020.
Heat shock protein beta 1 (), a member of the heat-shock family of protein, is a relatively small (27 kDa) molecular chaperone protein associated with cellular development. The relationship between expression and muscle growth in beef cattle has previously been reported, but there have been no reports of DNA markers related to meat quantity in Korean native steers. Therefore, the aim of this study was to evaluate the relationship of single-nucleotide polymorphisms (SNPs) within in terms of the carcass traits related to muscle growth in Korean native steers. Through direct sequencing, we discovered three SNPs: g.111 T > C SNP (rs208395876) and g.2548 C > G SNP (rs483014585) were respectively located in 5 UTR (untranslated region) and 3 UTR. Further, g.2352 T > C SNP (rs110832311) was located in the adjacent region of the RNA splicing site. The least square means of steers with a CC genotype of g.111 T > C SNP had a significantly higher meat ratio ( 0.04), while the least square means of steers with a CC genotype of g.2352 T > C SNP had a significantly higher meat ratio ( 0.002) and lower back-fat thickness ( 0.004) than those of the other genotype. Moreover, although the least square means of steers with CC-CC, CT-CC, and TT-CC genotypes were significantly decreased for back-fat thickness, they were significantly increased for the meat ratio. Therefore, our results suggested that g.111 T > C SNP and g.2352 T > C SNP could be a causal mutation related to an adipose metabolism in Korean cattle steer.
热休克蛋白β1（）是热休克蛋白家族的成员，是一种相对较小（27 kDa）的分子伴侣蛋白，与细胞发育相关。此前已有关于肉牛中该蛋白表达与肌肉生长关系的报道，但在韩牛阉牛中尚未有与肉量相关的DNA标记的报道。因此，本研究的目的是评估该基因内单核苷酸多态性（SNP）与韩牛阉牛肌肉生长相关胴体性状之间的关系。通过直接测序，我们发现了三个SNP：g.111 T>C SNP（rs208395876）和g.2548 C>G SNP（rs483014585）分别位于5′非翻译区（UTR）和3′UTR。此外，g.2352 T>C SNP（rs110832311）位于RNA剪接位点的相邻区域。g.111 T>C SNP的CC基因型阉牛的最小二乘均值具有显著更高 的肉比（P<0.04），而g.2352 T>C SNP的CC基因型阉牛的最小二乘均值与其他基因型相比具有显著更高的肉比（P<0.002）和更低的背膘厚度（P<0.004）。此外，虽然CC-CC、CT-CC和TT-CC基因型阉牛的最小二乘均值背膘厚度显著降低，但肉比显著增加。因此，我们的结果表明g.111 T>C SNP和g.2352 T>C SNP可能是与韩牛阉牛脂肪代谢相关的因果突变。
