Cunha Medeiros Aldo, Meneses Rego Amália, Medeiros Azevedo Italo, Ferreira Carvalho Marília, Brasil Medeiros Vítor, Araujo-Filho Irami
Department of Surgery, Federal University of Rio Grande do Norte, Natal, Brazil.
J Invest Surg. 2011;24(3):109-14. doi: 10.3109/08941939.2011.557468.
The Roux-en-Y gastric bypass (RYGB) may affect metabolism, microbiology, and histology of gastric remnant. Therefore, this study aimed to investigate these issues in rats.
Twelve rats were randomly allocated to a RYGB group (n = 6) and nonoperated normal rats group (n = 6). After 30 postoperative days, all rats were injected with 0.1 ml of Na(99m)Tc(-) i.v. (radioactivity 0.66 MBq). After 30 min, liver, stomach, thyroid, heart, lung, kidney, and femur samples were harvested and weighed. Percentage radioactivity per gram of organ (%ATI/g) was determined using a Perkin-Elmer gamma counter. Serum albumin, calcium, aminotransferases (ALT, AST), T3, T4, and PTH were determined. Samples of the excluded stomach mucosa were harvested for bacterial and fungal count such as colony-forming units/g and histology.
A significant reduction (t test) in %ATI/g was observed in the liver, stomach, and femur in the RYGB rats, compared with normal rats (p < .05). A significant reduction in serum albumin and calcium in RYGB rats was detected, compared with normal rats (p < .05). ALT and AST were significantly different between the two groups. T3 and T4 levels were significantly lower in RYGB rats than in normal rats (p < .05); PTH levels were higher in RYGB rats than in controls (p = .009). The gastric remnant mucosa of RYGB rats showed higher bacterial and fungal count, atrophy, intestinal metaplasia, and neutrophilic polymorphonuclear inflammation than in normal rats.
This investigation demonstrated that a model of murine RYGB significantly modified metabolic parameters and the microbiology/histology of the remnant stomach.
Roux-en-Y胃旁路术（RYGB）可能会影响胃残余部分的代谢、微生物学及组织学。因此，本研究旨在对大鼠的这些问题进行调查。
将12只大鼠随机分为RYGB组（n = 6）和未手术的正常大鼠组（n = 6）。术后30天，所有大鼠经静脉注射0.1 ml的99mTc-Na（放射性0.66 MBq）。30分钟后，采集肝脏、胃、甲状腺、心脏、肺、肾脏和股骨样本并称重。使用珀金埃尔默γ计数器测定每克器官的放射性百分比（%ATI/g）。测定血清白蛋白、钙、转氨酶（ALT、AST）、T3、T4和甲状旁腺激素（PTH）。采集被切除的胃黏膜样本进行细菌和真菌计数，如每克菌落形成单位，并进行组织学检查。
与正常大鼠相比，RYGB大鼠的肝脏、胃和股骨中的%ATI/g显著降低（t检验，p < 0.05）。与正常大鼠相比，RYGB大鼠的血清白蛋白和钙显著降低（p < 0.05）。两组之间的ALT和AST有显著差异。RYGB大鼠的T3和T4水平显著低于正常大鼠（p < 0.05）；RYGB大鼠的PTH水平高于对照组（p = 0.009）。与正常大鼠相比，RYGB大鼠的胃残余黏膜显示出更高的细菌和真菌计数、萎缩、肠化生以及嗜中性多形核炎症。
本研究表明小鼠RYGB模型显著改变了代谢参数以及残余胃的微生物学/组织学。
