袖状胃切除术后肥胖大鼠胃形态和组织病理学的短期和长期变化。
Short- and long-term changes in gastric morphology and histopathology following sleeve gastrectomy in diet-induced obese rats.
机构信息
Department of Histology & Pathology, University of Navarra, Pamplona, Spain.
出版信息
Obes Surg. 2012 Apr;22(4):634-40. doi: 10.1007/s11695-012-0606-3.
BACKGROUND
Sleeve gastrectomy (SG) has been used as a multipurpose surgical procedure for the treatment of morbid obesity. The aim of the study was to analyze gastric morphology and histology at two different time points after SG in rats.
METHODS
Thirty-five male Wistar rats were fed ad libitum during 3 months on a high-fat diet to induce obesity. Subsequently, 25 diet-induced obese rats underwent either SG (n = 12) or a sham operation (n = 13). The remaining ten obese animals encompassed the nonoperated control group (Co). Four weeks postoperatively, 15 rats (n = 5 rats/experimental group) were sacrificed, while the remaining 20 rats were sacrificed after 16 weeks (animals/group; Co = 5, sham = 8, SG = 7) to compare the gastric morphological and histopathological changes over time. Body weight and food intake were regularly recorded.
RESULTS
For both time periods, the Co groups exhibited the highest body weight, while the rats undergoing the SG showed the lowest weight gain (P < 0.05). Initially, significant differences (P < 0.005) in food intake relative to body weight were observed between the Co rats and animals undergoing surgery, which disappeared thereafter. The actual total stomach size after both experimental periods in the SG group was similar to that of non- and sham-operated rats mainly due to a forestomach enlargement, which was more pronounced after 16 weeks. Traits of gastritis cystica profunda characterized by gastric foveolae elongation with hyperplasia and cystic dilatation of the glands were observed in the residual stomachs of the sleeve-gastrectomized rats. These findings were mostly observed after 16 weeks of performing the SG, although they were also detected occasionally following 4 weeks postoperatively. No intestinal metaplasia was observed.
CONCLUSION
After SG gastric macro- and microscopic changes with functional implications in both the short and long term take place.
背景
袖状胃切除术（SG）已被用作治疗病态肥胖的多用途手术。本研究旨在分析大鼠 SG 后两个不同时间点的胃形态和组织学变化。
方法
35 只雄性 Wistar 大鼠在 3 个月内自由进食高脂肪饮食以诱导肥胖。随后，25 只饮食诱导肥胖大鼠行 SG（n = 12）或假手术（n = 13）。其余 10 只肥胖动物为未手术对照组（Co）。术后 4 周，15 只大鼠（n = 5 只/实验组）被处死，其余 20 只大鼠在 16 周后被处死（动物/组；Co = 5，假手术 = 8，SG = 7）以比较随时间的胃形态和组织病理学变化。定期记录体重和食物摄入量。
结果
对于两个时间段，Co 组的体重最高，而接受 SG 的大鼠体重增加最低（P < 0.05）。最初，Co 组大鼠与手术动物之间的相对体重的食物摄入量存在显著差异（P < 0.005），此后消失。SG 组两个实验期后实际总胃大小与非手术和假手术大鼠相似，主要是由于前胃增大，16 周后更为明显。在接受 SG 后 16 周，在袖状胃切除大鼠的残胃中观察到深部胃炎囊性变的特征，表现为胃小凹伸长，腺体囊性扩张和增生。这些发现主要发生在进行 SG 后 16 周，但术后 4 周偶尔也会检测到。未观察到肠上皮化生。
结论
SG 后胃的宏观和微观变化会在短期和长期产生功能影响。
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