URAFPA, INRA UC340, Nancy Université, BP 172, 54505 Vandoeuvre-lès-Nancy Cedex, France.
Toxicol Lett. 2011 Jul 4;204(1):57-63. doi: 10.1016/j.toxlet.2011.04.009. Epub 2011 Apr 15.
Polybrominated diphenyl ethers (PBDEs) are flame retardants. Because of their high lipophilicity and persistence, PBDEs bioaccumulate in all abiotic and biological matrices. The aim of this study was to investigate the long-term neurobehavioral and physiological effects of exposure to environmental doses of PBDE-99 in adult rats. Rats received a daily administration of PBDE-99 for 90 days by oral gavage at 0.15, 1.5 and 15μg/kg, doses which are relevant of human exposure. Before and after the 90 days of exposure, behavioral tests including the open-field and the elevated plus-maze tests for locomotor activity and anxiety, and the Morris water maze for spatial learning were conducted. Physiological measures such as body weight, food and water consumption, organs weight, hepatic enzymes levels and PBDE-99 concentration in adipose tissue were also evaluated at the end of exposure. There was no effect on body weight, food and water consumption, organs weight, hepatic enzymes levels despite rising PBDE-99 concentration in adipose tissue with the doses tested. Moreover, there was no effect on locomotor activity and exploration, and spatial learning. Deleterious effects of PBDE-99 at high doses have often been highlighted in many studies after an acute dose whereas exposure during 90 days at realistic doses would have no significant effect in adult rats.
多溴二苯醚（PBDEs）是阻燃剂。由于其高亲脂性和持久性，PBDEs 会在所有非生物和生物基质中生物累积。本研究旨在探讨成年大鼠长期暴露于环境剂量 PBDE-99 的神经行为和生理影响。大鼠通过口服灌胃每天接受 PBDE-99 处理 90 天，剂量分别为 0.15、1.5 和 15μg/kg，这些剂量与人类暴露相关。在暴露前和 90 天后，进行了行为测试，包括旷场和高架十字迷宫测试以评估运动活动和焦虑程度，以及 Morris 水迷宫测试以评估空间学习能力。在暴露结束时，还评估了体重、食物和水的消耗、器官重量、肝酶水平以及脂肪组织中 PBDE-99 的浓度等生理指标。尽管在测试剂量下脂肪组织中 PBDE-99 的浓度升高，但体重、食物和水的消耗、器官重量、肝酶水平均无影响。此外，运动活动和探索以及空间学习能力也未受影响。在许多研究中，急性高剂量的 PBDE-99 往往会产生有害影响，而在成年大鼠中，90 天的实际剂量暴露则没有显著影响。
