利用 Taq(α)I 内切酶进行联合糖基化限制分析（CGRA）检测 5-羟甲基胞嘧啶。
Detection of 5-hydroxymethylcytosine in a combined glycosylation restriction analysis (CGRA) using restriction enzyme Taq(α)I.
机构信息
Department of Chemistry and Institute for Biophysical Dynamics, The University of Chicago, Chicago, IL, USA.
出版信息
Bioorg Med Chem Lett. 2011 Sep 1;21(17):5075-7. doi: 10.1016/j.bmcl.2011.03.118. Epub 2011 Apr 9.
Abstract
5-Hydroxymethylcytosine (5-hmC) is a newly discovered DNA base in mammalian cells that is believed to be another important epigenetic modification. Here we report the use of a methylation-insensitive restriction enzyme Taq(α)I coupled with selective chemical labeling of 5-hmC in a combined glycosylation restriction analysis (CGRA) to detect 5-hmC in TCGA sequences. This method, differentiates fully versus hemi-hydroxymethylated cytosine in the CpG dinucleotide, adds a new tool to facilitate biological studies of 5-hmC.
摘要
5-羟甲基胞嘧啶（5-hmC）是哺乳动物细胞中一种新发现的 DNA 碱基，被认为是另一种重要的表观遗传修饰。在这里，我们报告了使用甲基化不敏感的限制酶 Taq(α)I 与 5-hmC 的选择性化学标记相结合的方法，即组合糖基化限制分析（CGRA），在 TCGA 序列中检测 5-hmC。该方法可区分 CpG 二核苷酸中的全羟甲基化胞嘧啶和半羟甲基化胞嘧啶，为 5-hmC 的生物学研究提供了新的工具。
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