Departamento de Bioquímica, Universidade Federal de Santa Catarina, Florianópolis, Brazil.
Libyan J Med. 2011 Jan 18;6. doi: 10.3402/ljm.v6i0.5651.
Bidens pilosa is a plant used by Amazonian and Asian folks for some hepatopathies. The hydroethanol crude extract and three fractions were assessed for antioxidant and hepatoprotective effects. Higher levels of scavenger activity on the 1,1-diphenyl-2-picrylhydrazyl radical, inhibition of deoxyribose oxidation and lipid peroxidation in vitro were detected for the ethyl acetate fraction (IC(50)4.3-32.3 µg/ml) followed by the crude extract (IC(50)14.2-98.0 µg/ml). The ethyl acetate fraction, again followed by the crude extract, showed high contents of total soluble polyphenols (3.6±0.2 and 2.1±0.2 GAE/mg, respectively) and presence of a quercetin-derived flavonoid identified as quercetin 3,3'-dimethyl ether 7-O-β-D-glycopyranoside. Both products were assayed for hepatoprotector effects against CCl(4)-induced liver injury in mice. Markers of oxidative stress and hepatic injury were evaluated. The results showed that the 10-day pretreatments (15 mg/kg, p.o.) protected the livers against injury by blocking CCl(4)-induced lipid peroxidation and protein carbonylation and the DNA fragmentation was decreased (~60%). The pretreatments avoided the loss of the plasma ferric reducing/antioxidant power and the elevation of serum transaminases and lactate dehydrogenase activities. The results suggest that the main constituents responsible for the hepatoprotective effects with free radical scavenger power associated are well extracted by performing fractionation with ethyl acetate. The findings support the Brazilian traditional use of this plant and justify further evaluations for the therapeutic efficacy and safety of the constituents of the ethyl acetate fraction to treat some liver diseases.
鬼针草是一种被亚马逊和亚洲人民用于治疗某些肝病的植物。评估了水-乙醇粗提物及其三个馏分的抗氧化和保肝作用。乙酸乙酯馏分（IC50 为 4.3-32.3μg/ml）对 1,1-二苯基-2-苦基肼基自由基具有更高的清除活性，体外抑制脱氧核糖氧化和脂质过氧化作用，其次是粗提物（IC50 为 14.2-98.0μg/ml）。再次是乙酸乙酯馏分，粗提取物表现出较高的总可溶性多酚含量（分别为 3.6±0.2 和 2.1±0.2 GAE/mg）和存在一种槲皮素衍生的黄酮类化合物，鉴定为槲皮素 3,3'-二甲醚 7-O-β-D-吡喃糖苷。两种产物均用于测定对 CCl4 诱导的小鼠肝损伤的保肝作用。评估了氧化应激和肝损伤的标志物。结果表明，10 天预处理（15mg/kg，po）通过阻断 CCl4 诱导的脂质过氧化和蛋白质羰基化来保护肝脏免受损伤，并且 DNA 片段化减少了约 60%。预处理避免了血浆铁还原/抗氧化能力的丧失和血清转氨酶和乳酸脱氢酶活性的升高。结果表明，用乙酸乙酯进行馏分分离可以很好地提取具有自由基清除能力的主要成分，从而发挥保肝作用。这些发现支持巴西传统上使用这种植物，并为进一步评估乙酸乙酯馏分的治疗功效和安全性以治疗某些肝脏疾病提供了依据。
