Department of Animal Biology and Physiology, Laboratory of Animal Physiology, University of Yaounde I, P.O. Box 812, Yaounde, Cameroon.
Faculty of Health Sciences, Université des Montagnes, P.O. Box 208, Bangangté, Cameroon.
BMC Complement Altern Med. 2017 Oct 10;17(1):479. doi: 10.1186/s12906-017-1972-0.
Essential hypertension is mainly caused by endothelial dysfunction which results from nitric oxide (NO) deficiency. The present study was design to evaluate the protective effect of Bidens pilosa ethylene acetate extract (Bp) on L-NAME induced hypertension and oxidative stress in rats.
Male Wistar rats were used to induce hypertension by the administration of L-NAME (a non-pecific nitric oxide inhibitor) (50 mg/kg/day). The others groups were receiving concomitantly L-NAME plus Bp extract (75 and 150 mg/kg/day) or losartan (25 mg/kg/day). All the treatments were given orally for 4 weeks. At the end of the treatment, the hemodynamic parameters were recorded using the direct cannulation method. The effects of the extract on lipid profile, kidney and liver functions as well as oxidative stress markers were evaluated by colorimetric method. Results were expressed as the mean ± SEM. The difference between the groups was compared using one-way analysis of variance (ANOVA) followed by the Duncan's post hoc test.
Animals receiving L-NAME presented high blood pressure, normal heart rate and lipid profile as well as NO depletion, liver and kidney injuries and oxidative stress. The concomitant treatment with L-NAME and Bp or losartan succeeded to prevent the raised of blood pressure and all the other injuries without affecting the heart rate.
These results confirm the antihypertensive effects of Bidens pilosa and highlight its protective properties in L-NAME model of hypertension in rat, probably due to the presence of Quercetin 3,3 '-dimethyl ether 7-0-β-D-glucopyranoside.
原发性高血压主要由内皮功能障碍引起，而内皮功能障碍是由于一氧化氮（NO）缺乏所致。本研究旨在评估三叶鬼针草乙酸乙酯提取物（Bp）对 L-NAME 诱导的高血压和大鼠氧化应激的保护作用。
雄性 Wistar 大鼠给予 L-NAME（一种非特异性一氧化氮抑制剂）（50mg/kg/天）以诱导高血压。其他组同时给予 L-NAME 加 Bp 提取物（75 和 150mg/kg/天）或氯沙坦（25mg/kg/天）。所有治疗均口服给药 4 周。治疗结束时，使用直接插管法记录血流动力学参数。通过比色法评估提取物对血脂谱、肝肾功能以及氧化应激标志物的影响。结果表示为平均值±SEM。使用单向方差分析（ANOVA）比较组间差异，然后使用 Duncan 事后检验进行比较。
接受 L-NAME 治疗的动物表现出高血压、正常心率和血脂谱以及 NO 耗竭、肝肾功能损伤和氧化应激。同时给予 L-NAME 和 Bp 或氯沙坦成功预防了血压升高和所有其他损伤，而不影响心率。
这些结果证实了三叶鬼针草的降压作用，并强调了其在 L-NAME 诱导的大鼠高血压模型中的保护特性，这可能归因于槲皮素 3,3'-二甲醚 7-O-β-D-吡喃葡萄糖苷的存在。
