Department of Infectious Diseases, Second Affiliated Hospital of Medical School of Xi'an Jiaotong University, 157 Xiwu Road, Xi'an 710004, Shaanxi Province, China.
World J Gastroenterol. 2011 Apr 21;17(15):2044-8. doi: 10.3748/wjg.v17.i15.2044.
To access the frequency and level of apoptotic CD34+ cells isolated from the marrow fluid of patients with post-hepatitis cirrhosis.
The frequency of bone marrow CD34+ cells and apoptotic bone marrow CD34+ cells in 31 in-patients with post-hepatitis cirrhosis (cirrhosis group), and 15 out-patients without liver or blood disorders (control group) was calculated by flow cytometry. Parameters were collected to evaluate liver functions of patients in cirrhosis group.
The percentage of normal bone marrow CD34+ cells was 6.30% ± 2.48% and 1.87% ± 0.53% (t = 3.906, P < 0.01) while that of apoptotic marrow CD34+ cells was 15.00% ± 15.81% and 5.73% ± 1.57% (t = 2.367, P < 0.05) in cirrhosis and control groups, respectively. The percentage of apoptotic marrow CD34+ cells was 6.25% ± 3.30% and 20.92 ± 18.5% (t = 2.409, P < 0.05) in Child-Pugh A and Child-Pugh B + C cirrhotic patients, respectively. The percentage of late apoptotic marrow CD34+ cells was positively correlated with the total bilirubin and aspartate aminotransferase serum levels in patients with cirrhosis.
The status of CD34+ marrow cells in cirrhotic patients may suggest that the ability of hematopoietic progenitor cells to transform into mature blood cells is impaired.
检测肝炎后肝硬化患者骨髓液中分离的 CD34+细胞的凋亡频率和水平。
采用流式细胞术检测 31 例肝炎后肝硬化住院患者（肝硬化组）和 15 例无肝、血病史的门诊患者（对照组）骨髓液中 CD34+细胞及凋亡的 CD34+细胞的频率。收集肝硬化组患者的肝功能参数。
正常骨髓 CD34+细胞百分比分别为 6.30%±2.48%和 1.87%±0.53%（t=3.906，P<0.01），凋亡的骨髓 CD34+细胞百分比分别为 15.00%±15.81%和 5.73%±1.57%（t=2.367，P<0.05）。肝硬化组中，Child-Pugh A 级和 Child-Pugh B+C 级患者的凋亡骨髓 CD34+细胞百分比分别为 6.25%±3.30%和 20.92%±18.5%（t=2.409，P<0.05）。肝硬化患者晚期凋亡骨髓 CD34+细胞百分比与总胆红素和天门冬氨酸转氨酶血清水平呈正相关。
肝硬化患者骨髓 CD34+细胞状态可能提示造血祖细胞向成熟血细胞转化的能力受损。
