Yatscoff R W, Goldstein S, Freeman K B
Somatic Cell Genet. 1978 Nov;4(6):633-45. doi: 10.1007/BF01543155.
Proteins synthesized in mitochondria of 27 different human cell lines, identified by labeling with [35S]methionine in the presence of cycloheximide, have been enumerated and their electrophoretic mobilities determined by sodium dodecyl sulfate-polyacrylamide slab gel electrophoresis and fluorography. Twelve bands were observed in all cell lines. In 24 cell lines, the electrophoretic mobilities of the proteins were the same regardless of race, sex, tissue of origin, cell type, viral transformation, or premature biological aging syndromes. The patterns obtained for the remaining cell lines, HeLa, KB, and Hep-2 were identical. These cell lines showed one protein component that was absent in the 24 others, and lacked a component present in these cell lines. Since it has been previously asserted that KB and Hep-2 are HeLa cells, the data indicate that one basic pattern exists in human cells with a variant of unknown origin occurring in HeLa cells.
在27种不同人类细胞系的线粒体中合成的蛋白质，通过在放线菌酮存在的情况下用[35S]甲硫氨酸标记来鉴定，已进行了计数，并通过十二烷基硫酸钠-聚丙烯酰胺平板凝胶电泳和荧光自显影测定了它们的电泳迁移率。在所有细胞系中均观察到12条带。在24种细胞系中，无论种族、性别、起源组织、细胞类型、病毒转化或早衰生物学综合征如何，蛋白质的电泳迁移率都是相同的。其余细胞系HeLa、KB和Hep-2所获得的图谱是相同的。这些细胞系显示出一种在其他24种细胞系中不存在的蛋白质成分，并且缺少这些细胞系中存在的一种成分。由于此前有人断言KB和Hep-2是HeLa细胞，数据表明人类细胞中存在一种基本模式，而HeLa细胞中出现了一种来源不明的变体。
