Division of Human Biology, International Medical University, 57000 Kuala Lumpur, Malaysia.
J Zhejiang Univ Sci B. 2011 May;12(5):335-45. doi: 10.1631/jzus.B1000336.
This study aimed to assess the inhibitory activities of methanol extracts from the microalgae Ankistrodesmus convolutus, Synechococcus elongatus, and Spirulina platensis against Epstein-Barr virus (EBV) in three Burkitt's lymphoma (BL) cell lines, namely Akata, B95-8, and P3HR-1. The antiviral activity was assessed by quantifying the cell-free EBV DNA using real-time polymerase chain reaction (PCR) technique. The methanol extracts from Ankistrodesmus convolutus and Synechococcus elongatus displayed low cytotoxicity and potent effect in reducing cell-free EBV DNA (EC(50)<0.01 µg/ml) with a high therapeutic index (>28000). After fractionation by column chromatography, the fraction from Synechococcus elongatus (SEF1) reduced the cell-free EBV DNA most effectively (EC(50)=2.9 µg/ml, therapeutic index>69). Upon further fractionation by high performance liquid chromatography (HPLC), the sub-fraction SEF1'a was most active in reducing the cell-free EBV DNA (EC(50)=1.38 µg/ml, therapeutic index>14.5). This study suggests that microalgae could be a potential source of antiviral compounds that can be used against EBV.
本研究旨在评估甲醇提取物对三种微藻（卷曲鱼腥藻、聚球藻和钝顶螺旋藻）对三种伯基特淋巴瘤（BL）细胞系（阿克塔、B95-8 和 P3HR-1）中 EBV 的抑制活性。采用实时聚合酶链反应（PCR）技术定量检测无细胞 EBV DNA 来评估抗病毒活性。卷曲鱼腥藻和聚球藻的甲醇提取物显示出低细胞毒性和显著降低细胞游离 EBV DNA 的作用（EC（50）<0.01μg/ml，治疗指数>28000）。经柱层析分离后，来自聚球藻（SEF1）的馏分最有效地降低了细胞游离 EBV DNA（EC（50）=2.9μg/ml，治疗指数>69）。经高效液相色谱（HPLC）进一步分离后，亚馏分 SEF1'a 最有效地降低了细胞游离 EBV DNA（EC（50）=1.38μg/ml，治疗指数>14.5）。本研究表明，微藻可能是一种潜在的抗病毒化合物来源，可用于对抗 EBV。
