Arun Peethambaran, Spadaro John, John Jennifer, Gharavi Robert B, Bentley Timothy B, Nambiar Madhusoodana P
Blast-Induced Neurotrauma Branch, Center for Military Psychiatry and Neuroscience, Walter Reed Army Institute of Research, Silver Spring, Maryland 20910, USA.
Neuroreport. 2011 Jun 11;22(8):379-84. doi: 10.1097/WNR.0b013e328346b138.
One of the major limitations in studying the mechanisms of blast-induced traumatic brain injury (bTBI) or screening therapeutics for protection is the lack of suitable laboratory model systems that can closely mimic the complex blast exposure. Although animal models of bTBI that use shock tubes to mimic blast exposure are available, no high throughput shock tube-based in-vitro models have been reported. Here, we report an in-vitro bTBI model using a compressed air-driven shock tube and mouse neuroblastoma/rat glioblastoma hybrid cells (NG108-15) or SH-SY5Y human neuroblastoma cells in tissue culture plates. Our data showed significant neurobiological effects with decreased adenosine triphosphate levels, increased cellular injury, lactate dehydrogenase release, and reactive oxygen species formation after blast exposure.
研究爆炸所致创伤性脑损伤（bTBI）机制或筛选保护性治疗方法的主要限制之一是缺乏能够紧密模拟复杂爆炸暴露的合适实验室模型系统。虽然有使用冲击管模拟爆炸暴露的bTBI动物模型，但尚未报道基于高通量冲击管的体外模型。在此，我们报告一种体外bTBI模型，该模型在组织培养板中使用压缩空气驱动的冲击管和小鼠神经母细胞瘤/大鼠胶质母细胞瘤杂交细胞（NG108-15）或SH-SY5Y人神经母细胞瘤细胞。我们的数据显示，爆炸暴露后三磷酸腺苷水平降低、细胞损伤增加、乳酸脱氢酶释放以及活性氧形成，具有显著的神经生物学效应。
