胸腔内注射间充质干细胞：胸膜疾病治疗的新策略。

Intrapleural delivery of mesenchymal stem cells: a novel potential treatment for pleural diseases.

机构信息

Department of Pulmonary Medicine, Huadong Hospital, Shanghai Medical College, Fudan University, Shanghai 200040, China.

出版信息

Acta Pharmacol Sin. 2011 May;32(5):581-90. doi: 10.1038/aps.2011.22. Epub 2011 May 2.

DOI:10.1038/aps.2011.22

PMID:21532612

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4002516/

Abstract

AIM

To develop a method to deliver mesenchymal stem cells (MSCs) into the pleural cavity for the treatment of pleural diseases.

METHODS

MSCs were isolated from rat bone marrow of rats and labeled with 4',6-diamidino-2-phenylindole dihydrochloride (DAPI) or green fluorescent protein (GFP) using a lentiviral vector. Eighteen Sprague-Dawley (SD) rats were inoculated intrapleurally with 1×10(6) MSCs-DAPI. The distribution of the fluorescent cells was observed using fluorescent microscopy for the following 30 d. Another 12 rats inoculated intrapleurally with 1×10(6) MSCs-GFP were observed for 14 d.

RESULTS

The isolated cells were typical MSC phenotypes and could differentiate into adipocytes, osteoblasts, and chondroblasts in vitro. Microscopic analysis revealed that the labeled cells adhered to the surface of the pleural cavity. The highest number of the labeled cells was found to be adhered to all specimens from the mediastinal pleura, but no labeled cells were detected in the lung parenchyma or other tissues/organs, such as the liver, kidney, spleen, and mesenterium. Incidentally, stomas were found in the mediastinal pleura. The recovered MSCs-GFP from the pleural cavity retained their ability to adhere and proliferate.

CONCLUSION

We have established a novel method for intrapleural delivery of MSCs. The distribution of intrapleurally delivered MSCs was found to be limited to the pleurae and the pleural cavity, thereby providing us with a new approach to further investigation of the therapeutic roles of MSCs in pleural diseases.

摘要

目的

开发一种将间充质干细胞（MSCs）递送至胸腔用于治疗胸膜疾病的方法。

方法

使用慢病毒载体从大鼠骨髓中分离 MSCs 并对其进行 4'，6-二脒基-2-苯基吲哚二盐酸盐（DAPI）或绿色荧光蛋白（GFP）标记。将 18 只 Sprague-Dawley（SD）大鼠通过胸膜内接种 1×10(6)个 MSC-DAPI。在接下来的 30 天内使用荧光显微镜观察荧光细胞的分布。另外 12 只通过胸膜内接种 1×10(6)个 MSC-GFP 的大鼠观察 14 天。

结果

分离的细胞为典型的 MSC 表型，并且可以在体外分化为脂肪细胞、成骨细胞和成软骨细胞。显微镜分析显示，标记的细胞附着在胸腔表面。在纵隔胸膜的所有标本中发现最多数量的标记细胞附着，但在肺实质或其他组织/器官（如肝、肾、脾和肠系膜）中未检测到标记细胞。顺便提一下，在纵隔胸膜上发现了小孔。从胸腔回收的 MSC-GFP 保留了其粘附和增殖的能力。