Section of Pathological Anatomy, Polytechnic University of the Marche Region, School of Medicine, United Hospitals, Torrette, Ancona, Italy.
Cell Oncol (Dordr). 2011 Jun;34(3):235-43. doi: 10.1007/s13402-011-0031-y. Epub 2011 Apr 30.
The aim was to examine the expression and localization of the five somatostatin receptors (termed SSTR1 to 5) in radical prostatectomies (RPs) from patients with prostatic adenocarcinoma (PCa) under complete androgen ablation (CAA) before operation.
The five SSTRs were evaluated in the epithelial, smooth muscle and endothelial cells of normal-looking epithelium (Nep), high-grade prostatic intraepithelial neoplasia (HGPIN) and PCa in 20 RPs with clinically detected PCa from patients under CAA. Twenty RPs with clinically detected PCa from hormonally untreated patients were used as control group.
Concerning the secretory cells (i) Membrane staining was seen for SSTR3 and SSTR4; the mean percentages of positive cells, higher in SSTR3 than in SSTR4, decreased sharply in HGPIN and PCa compared with Nep; the mean percentages in the androgen ablated group were 30% to 90% lower than in the untreated; (ii) Cytoplasmic staining was seen for all five SSTRs; the mean percentages of positive cells in Nep, HGPIN and PCa of the untreated group were similar, and in general as high as 80% or more; in the treated group, the Nep values were similar to those in the untreated, whereas the values in HGPIN and PCa were lower for SSTR1, three and five, with a decrease of 30% for SSTR1; (iii) Nuclear staining was seen with SSTR4 and SSTR5, the mean percentages for the former being much lower than for the latter; treatment affected both HGPIN and PCa, whose proportions of stained cells were 30% to 55% lower than in the untreated group. Cytoplasmic staining in the basal cells was seen for all five SSTRs, both in Nep and HGPIN. The values in the treated group were lower than in the other, the difference between the two group being in general comprised between 10% and 40%. Treatment did not affect SSTR staining in the smooth muscle and endothelial cells.
The present study expands our knowledge on the expression and localization of the five SSTRs in the prostate following CAA.
研究完全雄激素阻断（CAA）前接受根治性前列腺切除术（RP）的前列腺腺癌（PCa）患者的前列腺组织中五种生长抑素受体（SSTR1 至 5）的表达和定位。
在 20 例接受 CAA 的临床诊断为 PCa 的 RP 中，评估了正常外观上皮（Nep）、高级别前列腺上皮内瘤变（HGPIN）和 PCa 中的上皮细胞、平滑肌细胞和内皮细胞中这五种 SSTR。20 例接受激素治疗的 PCa 患者的 RP 作为对照组。
关于分泌细胞（i）SSTR3 和 SSTR4 存在膜染色；与 Nep 相比，SSTR3 的阳性细胞百分比更高，而在 HGPIN 和 PCa 中则急剧下降；CAA 组的平均百分比比未治疗组低 30%至 90%；（ii）所有五种 SSTR 均存在细胞质染色；未治疗组 Nep、HGPIN 和 PCa 的阳性细胞百分比相似，通常高达 80%或更高；在治疗组中，Nep 值与未治疗组相似，而 HGPIN 和 PCa 中 SSTR1、3 和 5 的值较低，SSTR1 降低了 30%；（iii）SSTR4 和 SSTR5 存在核染色，前者的平均百分比明显低于后者；治疗影响 HGPIN 和 PCa，其染色细胞的比例比未治疗组低 30%至 55%。五种 SSTR 均在 Nep 和 HGPIN 的基底细胞中存在细胞质染色。治疗组的数值低于其他组，两组之间的差异通常在 10%至 40%之间。治疗不影响平滑肌和内皮细胞中的 SSTR 染色。
本研究扩展了我们对 CAA 后前列腺中五种 SSTR 表达和定位的认识。
