Sinkeldam E J, Kuper C F, Bosland M C, Hollanders V M, Vedder D M
TNO-CIVO Toxicology and Nutrition Institute, Zeist, The Netherlands.
Cancer Res. 1990 Feb 15;50(4):1092-6.
A 3 x 3 factorial experiment was conducted to examine how dietary fiber (wheat bran) and fat (lard) interactively affect the genesis of N-methyl-N'-nitro-N-nitrosoguanidine-induced colon cancer in rats. Groups of 30 male 4-week-old Wistar rats were fed ad libitum one of nine experimental diets containing either 15 (low), 27.5 (medium), or 40% (high) energy as fat in combination with 0.7 (low), 2.2 (medium), or 3.8 g (high) fiber/100 kcal for a period of 37 weeks. After 4 weeks, each rat received a total of five weekly intrarectal instillations of 6 mg N-methyl-N'-nitro-N-nitrosoguanidine/kg. The highest colon carcinoma incidence and the highest total number of carcinomas of the colon were observed in the animals fed the medium-fat/medium-fiber diet. The highest number of polyps and a relatively high polyp incidence occurred in the animals on the high-fat/low-fiber diet. An enhancing effect of fat on both the tumor incidence and tumor multiplicity was clearly present for the low-fiber diets, whereas fat had no effect when the fiber content of the diet was high. In general, the results showed a nonlinear dose-response relationship for fiber and fat. These results indicate that both dietary fiber and fat affect colon carcinogenesis in a complex, interactive manner.
进行了一项3×3析因实验，以研究膳食纤维（麦麸）和脂肪（猪油）如何交互影响N-甲基-N'-硝基-N-亚硝基胍诱导的大鼠结肠癌的发生。将30只4周龄雄性Wistar大鼠分为几组，随意喂食九种实验饮食中的一种，这些饮食含有15%（低）、27.5%（中）或40%（高）的脂肪能量，并分别与0.7（低）、2.2（中）或3.8克（高）纤维/100千卡搭配，持续37周。4周后，每只大鼠每周接受共5次直肠内注入6毫克N-甲基-N'-硝基-N-亚硝基胍/千克。在喂食中脂肪/中纤维饮食的动物中观察到最高的结肠癌发病率和最高的结肠肿瘤总数。在高脂肪/低纤维饮食的动物中出现了最多的息肉和相对较高的息肉发病率。对于低纤维饮食，脂肪对肿瘤发病率和肿瘤多发性均有明显的增强作用，而当饮食中的纤维含量高时，脂肪则没有影响。总体而言，结果显示纤维和脂肪呈非线性剂量反应关系。这些结果表明，膳食纤维和脂肪均以复杂的交互方式影响结肠癌的发生。
