Department of Ophthalmology, Tohoku University Graduate School of Medicine, Sendai, Japan.
Tissue Eng Part A. 2011 Sep;17(17-18):2213-9. doi: 10.1089/ten.TEA.2010.0568. Epub 2011 Jul 11.
We examined the feasibility of using gelatin hydrogels as carrier sheets for the transplantation of cultivated corneal endothelial cells. The mechanical properties, transparency, and permeability of gelatin hydrogel sheets were compared with those of atelocollagen sheets. Immunohistochemistry (ZO-1, Na(+)/K(+)-ATPase, and N-cadherin), hematoxylin and eosin staining, and scanning electron microscopy were performed to assess the integrity of corneal endothelial cells that were cultured on gelatin hydrogel sheets. The gelatin hydrogel sheets displayed greater transparency, elastic modulus, and albumin permeability compared to those of atelocollagen sheets. The corneal endothelial cells on gelatin hydrogel sheets showed normal expression levels of ZO-1, Na(+)/K(+)-ATPase, and N-cadherin. Hematoxylin and eosin staining revealed the formation of a continuous monolayer of cells attached to the gelatin hydrogel sheet. Scanning electron microscopy observations showed that the corneal endothelial cells were arranged in a regular, mosaic, and polygonal pattern with normal cilia. These results indicate that the gelatin hydrogel sheet is a promising material to transport corneal endothelial cells during transplantation.
我们研究了使用明胶水凝胶作为培养的角膜内皮细胞移植载体的可行性。比较了明胶水凝胶片的机械性能、透明度和通透性与去端肽胶原片的机械性能、透明度和通透性。通过免疫组织化学（ZO-1、Na(+)/K(+)-ATP 酶和 N-钙粘蛋白）、苏木精和伊红染色以及扫描电子显微镜评估培养在明胶水凝胶片上的角膜内皮细胞的完整性。与去端肽胶原片相比，明胶水凝胶片显示出更高的透明度、弹性模量和白蛋白通透性。明胶水凝胶片上的角膜内皮细胞 ZO-1、Na(+)/K(+)-ATP 酶和 N-钙粘蛋白的表达水平正常。苏木精和伊红染色显示出细胞与明胶水凝胶片附着形成连续的单层。扫描电子显微镜观察表明，角膜内皮细胞呈规则的、镶嵌的、多边形排列，纤毛正常。这些结果表明，明胶水凝胶片是一种有前途的在移植过程中运输角膜内皮细胞的材料。
