Suda T, Tozawa F, Ushiyama T, Sumitomo T, Yamada M, Demura H
Department of Medicine, Tokyo Women's Medical College, Japan.
Endocrinology. 1990 Feb;126(2):1223-8. doi: 10.1210/endo-126-2-1223.
To examine the effect of interleukin-1 (IL-1) on CRF and POMC gene expression, recombinant human IL-1 alpha and -beta were ip injected in rats. The plasma ACTH level showed a dose-related increase at 2 h after the injection of 0.5 and 2 micrograms IL-1 alpha and -beta, and also showed a sustained increase from 1 h until 5 h after the injection of 2 micrograms of IL-1 beta. CRF contents in the medial basal hypothalamus and ACTH contents in the anterior pituitary (AP) decreased at 2 h after the injection of 2 micrograms of IL-1 alpha and -beta, and such decreased levels were maintained until 5 h after the injection of 2 micrograms of IL-1 beta. The levels of CRF mRNA in the hypothalamus and POMC mRNA in AP significantly increased 3 h after the injection of 2 micrograms IL-1 alpha and -beta, and these levels were still higher at 5 h after the injection of 2 micrograms of IL-1 beta compared with those of the control. There was no significant change in the ACTH content and POMC mRNA levels in the intermediate-posterior pituitary or the hypothalamus or in the CRF contents and CRF mRNA levels in the cerebral cortex. These results indicate that acute administration of IL-1 alpha and -beta stimulates gene expression of hypothalamic CRF and CRF release, which causes the stimulation of ACTH release and POMC gene expression in AP.
为研究白细胞介素-1（IL-1）对促肾上腺皮质激素释放因子（CRF）和阿黑皮素原（POMC）基因表达的影响，将重组人IL-1α和IL-1β腹腔注射到大鼠体内。注射0.5微克和2微克IL-1α及IL-1β后2小时，血浆促肾上腺皮质激素（ACTH）水平呈剂量依赖性升高；注射2微克IL-1β后，血浆ACTH水平从1小时至5小时持续升高。注射2微克IL-1α及IL-1β后2小时，内侧基底下丘脑的CRF含量及垂体前叶（AP）的ACTH含量降低，且在注射2微克IL-1β后5小时仍维持在较低水平。注射2微克IL-1α及IL-1β后3小时，下丘脑CRF mRNA水平及AP的POMC mRNA水平显著升高，与对照组相比，在注射2微克IL-1β后5小时这些水平仍较高。垂体中间部-后部、下丘脑的ACTH含量及POMC mRNA水平，以及大脑皮质的CRF含量和CRF mRNA水平均无显著变化。这些结果表明，急性给予IL-1α和IL-1β可刺激下丘脑CRF的基因表达及CRF释放，进而刺激AP中ACTH释放及POMC基因表达。
