Moulin S, Fruchart J C, Dewailly P, Sezille G
Clin Chim Acta. 1979 Jan 15;91(2):159-63. doi: 10.1016/0009-8981(79)90451-0.
We describe a modified lipoprotein electrophoresis on acrylamide gel. The lipoproteins, prestained with Sudan Black, are separated by running on acrylamide-agarose gels, with two different concentrations of acrylamide and a constant concentration of agarose. The sera are deposited in the first gel (2% acrylamide). Chylomicrons remain at origin and other lipoproteins run through the first gel to the second one (3% acrylamide gel). VLDL stays at the junction of both gels, LDL and HDL are separated in the 3% acrylamide gel. By this technique, we were able to detect an increase of Lp(a) and to identify the different types of hyperlipoproteinemia. After running, the plates can be treated for storage.
我们描述了一种改良的丙烯酰胺凝胶脂蛋白电泳方法。用苏丹黑预染的脂蛋白，通过在含有两种不同浓度丙烯酰胺和恒定浓度琼脂糖的丙烯酰胺 - 琼脂糖凝胶上进行电泳来分离。血清加样于第一块凝胶（2%丙烯酰胺）。乳糜微粒留在原点，其他脂蛋白穿过第一块凝胶进入第二块凝胶（3%丙烯酰胺凝胶）。极低密度脂蛋白（VLDL）停留在两块凝胶的交界处，低密度脂蛋白（LDL）和高密度脂蛋白（HDL）在3%丙烯酰胺凝胶中分离。通过这种技术，我们能够检测到脂蛋白（a）[Lp(a)]的增加，并识别不同类型的高脂蛋白血症。电泳结束后，凝胶板可进行处理以便保存。
