Eklund A, Sjöblom L
Biochim Biophys Acta. 1986 Jun 11;877(1):135-40. doi: 10.1016/0005-2760(86)90128-1.
The use of a conventional agarose gel electrophoretic method to separate rat plasma lipoproteins resulted in a rather poor resolution of lipoproteins of lower density. Therefore, attempts were made to improve the resolution by running the electrophoresis under different conditions. It was shown that rat plasma lipoproteins could be separated into at least three fractions by agarose gel electrophoresis in phosphate buffer at pH 7.0. Using rat plasma lipoproteins isolated by sequential flotation as standards, these fractions were shown to correspond to high-density lipoprotein (HDL2), very-low-density lipoprotein (VLDL) and a mixed low-density lipoprotein (LDL)/high-density lipoprotein (HDL1) fraction. Since VLDL is completely separated from the other lipoprotein classes the method could be used to monitor changes in plasma VLDL.
使用传统的琼脂糖凝胶电泳方法分离大鼠血浆脂蛋白，导致低密度脂蛋白的分辨率相当差。因此，人们尝试通过在不同条件下进行电泳来提高分辨率。结果表明，在pH 7.0的磷酸盐缓冲液中，通过琼脂糖凝胶电泳可将大鼠血浆脂蛋白至少分离为三个组分。以通过连续浮选分离的大鼠血浆脂蛋白作为标准品，这些组分分别对应于高密度脂蛋白（HDL2）、极低密度脂蛋白（VLDL）以及低密度脂蛋白（LDL）/高密度脂蛋白（HDL1）混合组分。由于VLDL与其他脂蛋白类别完全分离，该方法可用于监测血浆VLDL的变化。
