Nauck M, Winkler K, März W, Wieland H
Department of Medicine, University Hospital of Freiburg, Germany.
Clin Chem. 1995 Dec;41(12 Pt 1):1761-7.
Quantification of lipoprotein cholesterol was performed by enzymatic staining of cholesterol in a new agarose gel electrophoresis method that allows the separation of LDL, VLDL, HDL, and lipoprotein(a) [Lp(a)]. Lp(a) shows an electrophoretic mobility clearly distinct from VLDL and HDL. The total CVs of lipoprotein cholesterol varied between 2.7% and 3.9% for LDL, 7.8% and 23.2% for VLDL, 5.2% and 9.5% for HDL, and 6.8% and 16.4% for Lp(a). Comparison of LDL-, VLDL-, and HDL-cholesterol concentrations with the results of a combined ultracentrifugation and precipitation technique gave correlation coefficients of 0.961, 0.947, and 0.918, respectively; comparison of Lp(a)-cholesterol values with those of a nephelometric Lp(a) assay gave r = 0.906. The new electrophoretic assay has several advantages: It allows the quantification of Lp(a)-cholesterol; VLDL-cholesterol is not affected by Lp(a)-cholesterol; and the LDL-cholesterol fraction does not contain Lp(a)-cholesterol, as happens with LDL-cholesterol determined by ultracentrifugation and precipitation.
采用一种新的琼脂糖凝胶电泳法，通过对胆固醇进行酶染色来定量脂蛋白胆固醇，该方法可分离低密度脂蛋白（LDL）、极低密度脂蛋白（VLDL）、高密度脂蛋白（HDL）和脂蛋白（a）[Lp（a）]。Lp（a）的电泳迁移率明显不同于VLDL和HDL。脂蛋白胆固醇的总变异系数在LDL为2.7%至3.9%、VLDL为7.8%至23.2%、HDL为5.2%至9.5%、Lp（a）为6.8%至16.4%之间。将LDL、VLDL和HDL胆固醇浓度与超速离心和沉淀联合技术的结果进行比较，相关系数分别为0.961、0.947和0.918；将Lp（a）胆固醇值与散射比浊法测定的Lp（a）值进行比较，r = 0.906。这种新的电泳测定法有几个优点：它可对Lp（a）胆固醇进行定量；VLDL胆固醇不受Lp（a）胆固醇影响；LDL胆固醇组分不含Lp（a）胆固醇，而超速离心和沉淀法测定的LDL胆固醇则会出现这种情况。
