Weima S M, van Rooijen M A, Mummery C L, Feyen A, de Laat S W, van Zoelen E J
Hubreht Laboratory, Netherlands Institute for Developmental Biology, The Netherlands.
Exp Cell Res. 1990 Feb;186(2):324-31. doi: 10.1016/0014-4827(90)90312-x.
The human embryonal carcinoma (EC) cell line Tera 2 clone 13 (T2/13) can be induced to differentiate in vitro into neuroectodermal cell types with retinoic acid. Undifferentiated cells are characterized by rapid proliferation, whereas differentiated cells show a prolonged generation time, have a limited life span, and possess new cell-surface markers. In the present study we establish that both differentiated and undifferentiated T2/13 cells express the type-B platelet-derived growth factor (PDGF) receptor mRNA and bind PDGF-BB with high affinity. Differentiation causes a three-fold increase in receptor number per cell and leaves the affinity of the receptors unaffected. These data are the first to describe expression of this receptor in EC cells. The biosynthesis and degradation of this receptor were studied in undifferentiated as well as in differentiated T2/13 cells using an anti-type-B receptor antibody. These experiments revealed that high concentrations of recombinant PDGF-AA did not accelerate receptor metabolism in both cell types. In contrast, human PDGF or recombinant PDGF-BB added to the culture dishes readily increased receptor degradation. These results demonstrate that T2/13 cells express functional type-B PDGF receptors and suggest that cells responsive to PDGF might be present during mammalian development before the onset of mesoderm formation.
人胚胎性癌（EC）细胞系Tera 2克隆13（T2/13）可在体外被视黄酸诱导分化为神经外胚层细胞类型。未分化细胞的特征是快速增殖，而分化细胞的代时延长，寿命有限，并具有新的细胞表面标志物。在本研究中，我们证实分化和未分化的T2/13细胞均表达B型血小板衍生生长因子（PDGF）受体mRNA，并以高亲和力结合PDGF-BB。分化使每个细胞的受体数量增加三倍，而受体的亲和力不受影响。这些数据首次描述了该受体在EC细胞中的表达。使用抗B型受体抗体，在未分化以及分化的T2/13细胞中研究了该受体的生物合成和降解。这些实验表明，高浓度的重组PDGF-AA在两种细胞类型中均未加速受体代谢。相反，添加到培养皿中的人PDGF或重组PDGF-BB很容易增加受体降解。这些结果表明T2/13细胞表达功能性B型PDGF受体，并提示在中胚层形成开始之前的哺乳动物发育过程中可能存在对PDGF有反应的细胞。
