Mummery C L, Feijen A, Moolenaar W H, van den Brink C E, de Laat S W
Exp Cell Res. 1986 Jul;165(1):229-42. doi: 10.1016/0014-4827(86)90547-1.
Aggregation of pluripotent P19 embryonal carcinoma (EC) cells in the presence of DMSO induces differentiation to various mesodermal cell types, including spontaneously contracting muscle. We have established clonal cell lines from these cultures and characterized one (MES-1) in particular for its response to growth factors. In contrast to the undifferentiated stem cells, but as a number of myoblast and muscle cell lines, MES-1 cells respond to both carbachol and bradykinin by the rapid release of Ca2+ from intracellular stores. In addition, MES-1 express receptors for and respond mitogenically to epidermal growth factor (EGF) and platelet-derived growth factor (PDGF). Isolated membranes from these cells retain the capacity to bind both ligands; addition of EGF to membranes induces endogenous phosphorylation of several proteins, including the EGF receptor itself and a 38 kD protein, while addition of PDGF specifically induces phosphorylation of the PDGF receptor. By contrast, other derivatives of P19, isolated from retinoic acid (RA)-treated aggregates and resembling neuroectodermal or endodermal cell types respond only to EGF; PDGF neither binds nor induces phosphorylation and a mitogenic response in these cells. During differentiation from EC cells therefore MES-1 cells developed a combination of growth factor receptor characteristics typical of somatic mesodermal cells and indicate that such receptors on EC-derived mesodermal cells are also functional.
在二甲基亚砜（DMSO）存在的情况下，多能性P19胚胎癌细胞聚集会诱导其分化为多种中胚层细胞类型，包括自发收缩的肌肉细胞。我们从这些培养物中建立了克隆细胞系，并特别对其中一个（MES-1）对生长因子的反应进行了表征。与未分化的干细胞不同，但与许多成肌细胞和肌肉细胞系一样，MES-1细胞对卡巴胆碱和缓激肽的反应是通过从细胞内储存中快速释放Ca2+。此外，MES-1表达表皮生长因子（EGF）和血小板衍生生长因子（PDGF）的受体，并对其有促有丝分裂反应。从这些细胞中分离出的膜保留了结合这两种配体的能力；向膜中添加EGF会诱导几种蛋白质的内源性磷酸化，包括EGF受体本身和一种38 kD的蛋白质，而添加PDGF则特异性地诱导PDGF受体的磷酸化。相比之下，从视黄酸（RA）处理的聚集体中分离出的、类似于神经外胚层或内胚层细胞类型的其他P19衍生物仅对EGF有反应；PDGF在这些细胞中既不结合也不诱导磷酸化和促有丝分裂反应。因此，在从胚胎癌细胞分化过程中，MES-1细胞形成了体细胞中胚层细胞典型的生长因子受体特征组合，这表明胚胎癌细胞衍生的中胚层细胞上的此类受体也是有功能的。
