Lab. of Biofunctional Material Chemistry, Faculty of Fisheries Sciences, Hokkaido Univ. Minato-cho 3-1-1, Hakodate, Hokkaido 041-8611, Japan.
J Food Sci. 2011 Jan-Feb;76(1):C104-11. doi: 10.1111/j.1750-3841.2010.01915.x. Epub 2010 Nov 29.
Japanese edible brown seaweeds, Eisenia bicyclis (Arame), Kjellmaniella crassifolia (Gagome), Alaria crassifolia (Chigaiso), Sargassum horneri (Akamoku), and Cystoseira hakodatensis (Uganomoku) were assayed for total phenolic content (TPC), fucoxanthin content, radical scavenging activities (DPPH, peroxyl radical, ABTS, and nitric oxide), and antioxidant activity in a liposome system. Among the solvents used for extraction, methanol was the most effective to extract total phenolics (TPC) from brown seaweeds. Among 5 kinds of brown seaweeds analyzed, methanol extract from C. hakodatensis was the best source for antioxidants. The high antioxidant activity of the extract was based not only on the high content of phenolics, but on the presence of fucoxanthin. No significant correlation (P > 0.05) was observed between TPC per gram extract with DPPH radical scavenging activity of the methanol extracts. These observed discrepancy would be due to structural variations in the phenolic compounds, and different levels of fucoxanthin in the extracts. The present study also demonstrated the synergy in the antioxidant activity of the combination of brown seaweed phenolics and fucoxanthin.
日本食用褐藻，包括双叉弯角藻（海蕴）、长松萝（昆布）、阔海带（裙带菜）、亨氏马尾藻（甘紫菜）和羊栖菜（海大麦），其总酚含量（TPC）、岩藻黄质含量、自由基清除活性（DPPH、过氧自由基、ABTS 和一氧化氮）以及在脂质体系统中的抗氧化活性进行了测定。在用于提取的溶剂中，甲醇是从褐藻中提取总酚（TPC）最有效的溶剂。在所分析的 5 种褐藻中，甲醇提取物来自羊栖菜是抗氧化剂的最佳来源。提取物的高抗氧化活性不仅基于酚类物质的高含量，而且还基于岩藻黄质的存在。甲醇提取物的 DPPH 自由基清除活性与每克提取物中的 TPC 之间没有显著相关性（P>0.05）。这种观察到的差异可能是由于酚类化合物的结构变化以及提取物中岩藻黄质的水平不同。本研究还证明了褐藻酚类和岩藻黄质的组合在抗氧化活性方面具有协同作用。
