新型单克隆抗体可与野生型和固定型狂犬病病毒株结合。

Novel monoclonal antibodies that bind to wild and fixed rabies virus strains.

机构信息

Laboratório de Imunologia Aplicada, Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de Santa Catarina, 88040-970, Florianópolis, SC, Brazil.

出版信息

J Virol Methods. 2011 Jul;175(1):66-73. doi: 10.1016/j.jviromet.2011.04.019. Epub 2011 Apr 28.

DOI:10.1016/j.jviromet.2011.04.019

PMID:21536074

Abstract

Ten monoclonal antibodies (MAbs) against rabies virus, including IgG3κ, IgG2aκ, IgMκ, and an IgG2bκ isotype, were produced and characterized using neutralization, ELISA, immunodot-blot, and immunofluorescence assays. MAb 8D11, which recognized rabies virus glycoprotein, was found to neutralize rabies virus in vitro. When submitted to an immunofluorescence assay, seven MAbs showed different reactivity against 35 Brazilian rabies virus isolates. Three MAbs (LIA 02, 3E6, and 9C7) only failed to recognize one or two virus isolates, whereas MAb 6H8 was found to be reactive against all virus isolates tested. MAbs were also evaluated for their immunoreactivity against fixed rabies virus strains present in human and veterinary commercial vaccines. MAbs LIA 02, 6H8, and 9C7 reacted against all vaccine strains, while the remaining MAbs recognized at least 76% of vaccine strains tested. This research provides a set of MAbs with potential application for improving existing or developing new diagnostic tests and immunoassays.

摘要

利用中和试验、ELISA、免疫斑点印迹和免疫荧光试验，制备并鉴定了 10 株针对狂犬病病毒的单克隆抗体（MAbs），包括 IgG3κ、IgG2aκ、IgMκ 和 IgG2bκ 同种型。MAb 8D11 识别狂犬病病毒糖蛋白，被发现能够在体外中和狂犬病病毒。当提交给免疫荧光试验时，7 株 MAb 对 35 株巴西狂犬病病毒分离株表现出不同的反应性。3 株 MAb（LIA 02、3E6 和 9C7）仅未能识别一个或两个病毒分离株，而 MAb 6H8 被发现对所有测试的病毒分离株均有反应性。MAb 还针对人用和兽用商业疫苗中存在的固定狂犬病病毒株进行了免疫反应性评估。MAb LIA 02、6H8 和 9C7 与所有疫苗株反应，而其余 MAb 则至少识别 76%的测试疫苗株。这项研究提供了一组 MAb，它们具有潜在的应用价值，可以改进现有的或开发新的诊断测试和免疫分析。