Pillai M R, Lo J M, Troutner D E
Department of Chemistry, University of Missouri, Columbia 65211.
Int J Rad Appl Instrum A. 1990;41(1):69-73. doi: 10.1016/0883-2889(90)90132-z.
Labeling of hematoporphyrin with 105Rh at stoichiometric concentrations is described. Labeling efficiencies of up to 93% could be obtained at pH 9.0 in bicarbonate buffer. Solvent extraction of 105Rh-hematoporphyrin into methyl isobutyl ketone was used to estimate the complex yield. The complex showed high stability and no loss of 105Rh was seen throughout the 6 days of study. 105Rh-hematoporphyrin when incubated with human gamma globulin was seen to be quantitatively bound to the protein. This procedure may be used for labeling monoclonal antibodies with 105Rh for therapeutic applications.
描述了在化学计量浓度下用¹⁰⁵铑标记血卟啉的方法。在碳酸氢盐缓冲液中pH 9.0时，标记效率可达93%。用甲基异丁基酮对¹⁰⁵铑 - 血卟啉进行溶剂萃取以估计络合物产率。该络合物显示出高稳定性，在整个6天的研究中未见¹⁰⁵铑损失。¹⁰⁵铑 - 血卟啉与人γ球蛋白孵育时，可定量结合到该蛋白质上。该方法可用于用¹⁰⁵铑标记单克隆抗体以用于治疗应用。
