Pillai M R, Lo J M, John C S, Troutner D E
Department of Chemistry, University of Missouri, Columbia 65211.
Int J Rad Appl Instrum B. 1990;17(4):419-26. doi: 10.1016/0883-2897(90)90111-d.
A bifunctional ligand 4-(p-aminobenzyl)-diethylenetriamine has been synthesized. 105Rh complexes with this ligand were prepared with an overall yield of 79% at pH 9.0 in bicarbonate buffer. The preformed complex was converted to the isothiocyanate derivative using thiophosgene. Conjugation yields of 75% with IgG and 85% with HSA could be obtained for a 4 h conjugation reaction. Affinity chromatography of human-IgG coupled to the rhodium complex in an anti-human IgG agarose gel indicated no denaturation of the labeled protein. The procedure reported here can be adapted for the preparation of 105Rh-labeled antibodies for therapeutic applications.
已合成一种双功能配体4-(对氨基苄基)-二亚乙基三胺。在碳酸氢盐缓冲液中pH 9.0条件下制备了该配体的¹⁰⁵Rh配合物，总产率为79%。使用硫光气将预先形成的配合物转化为异硫氰酸酯衍生物。对于4小时的偶联反应，与IgG的偶联产率可达75%，与HSA的偶联产率可达85%。在抗人IgG琼脂糖凝胶中对与人IgG偶联的铑配合物进行亲和层析，结果表明标记蛋白未发生变性。本文报道的方法可适用于制备用于治疗应用的¹⁰⁵Rh标记抗体。
