Isolation of human capillary endothelial cells from abdominal adipose tissue.
作者信息
Springhorn Jeremy P
出版信息
Cold Spring Harb Protoc. 2011 May 1;2011(5):pdb.prot4537. doi: 10.1101/pdb.prot4537.
PMID:21536769
Abstract
This protocol outlines a technique to isolate human capillary endothelial cells from human abdominal wall adipose tissue. The relative lack of cell-type complexity within fat tissue makes it an attractive source of endothelial cells. In addition, collaboration with a local plastic surgeon can yield significant quantities of subcutaneous adipose tissue from liposuction without the need to be on call for cadaveric or post-surgical samples.
摘要
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