表皮中超氧化物歧化酶活性的分布：采用电子自旋共振自旋捕集法进行测量
Distribution of superoxide dismutase activity in the epidermis: measurement with electron spin resonance spin trapping.
作者信息
Ogura R, Ueta H, Sugiyama M, Hidaka T
机构信息
Department of Medical Biochemistry, Kurume University School of Medicine, Japan.
出版信息
J Invest Dermatol. 1990 Feb;94(2):227-9. doi: 10.1111/1523-1747.ep12874551.
Superoxide dismutase (SOD) activity in cow snout epidermis was determined by the method of electron spin resonance (ESR) using the 5, 5 dimethyl-1-pyrroline-N-oxide (DMPO) spin trapping agent. The procedure was found to be a reliable measurement as compared with the ordinary method using xanthine oxidase NBT. SOD activity was distributed through the whole epidermis. This activity was higher in the lower layer than in the upper and middle layers.
采用电子自旋共振（ESR）方法，使用5,5-二甲基-1-吡咯啉-N-氧化物（DMPO）自旋捕获剂测定牛鼻表皮中的超氧化物歧化酶（SOD）活性。与使用黄嘌呤氧化酶NBT的常规方法相比，该方法被发现是一种可靠的测量方法。SOD活性分布于整个表皮。该活性在下层高于上层和中层。
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