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[采用电子自旋共振-自旋捕获法测定正常皮肤中超氧化物歧化酶活性]

[Measurement of superoxide dismutase activity in normal skin by electron spin resonance-spin trapping method].

作者信息

Yamagishi R, Ichihara T, Nagano G, Kamide R

出版信息

Nihon Hifuka Gakkai Zasshi. 1989 Feb;99(2):163-6.

PMID:2545959
Abstract

Superoxide dismutase (SOD) activity in 20 skin specimens from healthy individuals measured by the electron spin resonance-spin trapping method was 7.08 U +/- 0.41 U/mg protein (mean +/- SE). This method may be useful in the determination of SOD, since it requires a shorter time (approx. 3 min) and a smaller amount of specimen (approx. 30 mg) than previously reported methods other than the EIA method.

摘要

采用电子自旋共振-自旋捕集法测定20份健康个体皮肤标本中的超氧化物歧化酶(SOD)活性为7.08 U +/- 0.41 U/mg蛋白(均值 +/- 标准误)。该方法在SOD测定中可能有用,因为与除酶免疫分析方法外先前报道的方法相比,它所需时间更短(约3分钟)且标本用量更少(约30毫克)。

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