马缨丹叶提取物的细胞毒性和口服急性毒性研究。

Cytotoxicity and oral acute toxicity studies of Lantana camara leaf extract.

机构信息

Department of Biotechnology, Faculty of Applied Sciences, AIMST University, Jalan Bedong-Semeling, Batu 3 ½, Bukit Air Nasi, Bedong 08100, Kedah, Malaysia.

出版信息

Molecules. 2011 May 3;16(5):3663-74. doi: 10.3390/molecules16053663.

BACKGROUND

The objective of this study was to investigate the toxicity of Lantana camara methanol extract.

METHODS

In order to evaluate the toxicity of Lantana camara, the acute toxicity of the methanolic extract on adult mice and cytotoxicity test on Vero cell line were investigated. A fixed large dose of 2 g/kg body weight of L. camara leaf extract was administrated by a single oral gavage according to the OECD procedure.

RESULTS

In 2 weeks, L. camara leaf extract showed no obvious acute toxicity. While female mice lost body weight after being treated with single dose of leaf extract in acute toxicity test, male ones lost organ mass, particularly for heart and kidney. The biochemical liver function tests showed significantly elevated TBIL and ALT in the L. camara leaf extract treated female mice group compared with the control group. Cytotoxicity effect of leaf extract of L. camara was estimated through a MTT assay. Cytotoxicity tests on Vero cell line disclosed that leaf extract at concentrations up to 500 µg/mL inhibited the growth of cells 2.5 times less than did Triton 100 × 1%. More interestingly, the cytotoxicity initiated to decline at elevated concentrations of this extract.

CONCLUSIONS

The results of both tests confirm that L. camara shows a pro toxic effect.

背景

本研究旨在探究马缨丹甲醇提取物的毒性。

方法

为评估马缨丹的毒性，我们对成年小鼠进行了马缨丹甲醇提取物的急性毒性试验和对非洲绿猴肾细胞（Vero 细胞）的细胞毒性试验。根据 OECD 程序，我们采用固定大剂量 2 g/kg 体重对马缨丹叶片提取物进行单次口服灌胃。

结果

在 2 周内，马缨丹叶片提取物未表现出明显的急性毒性。虽然在急性毒性试验中，单次给予马缨丹叶片提取物后雌性小鼠体重减轻，但雄性小鼠的器官质量（尤其是心脏和肾脏）下降。生化肝功能检查显示，与对照组相比，马缨丹叶片提取物处理的雌性小鼠组的总胆红素（TBIL）和丙氨酸氨基转移酶（ALT）显著升高。通过 MTT 法评估马缨丹叶片提取物的细胞毒性作用。Vero 细胞系的细胞毒性试验表明，叶片提取物在高达 500 µg/mL 的浓度下对细胞的生长抑制作用比 Triton 100 × 1%低 2.5 倍。更有趣的是，随着提取物浓度的升高，其细胞毒性开始下降。