Beijing National Laboratory for Molecular Sciences, Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University, China.
Analyst. 2011 Jun 21;136(12):2613-8. doi: 10.1039/c0an01047b. Epub 2011 May 3.
Adulteration of herbal supplements with synthetic drugs is illegal. A rapid and reliable method which utilizes direct analysis in real time mass spectrometry (DART-MS) was developed for the identification of seven synthetic antidiabetic drugs used as adulterants in herbal dietary supplements. The supplement sample was simply extracted with methanol/water by manually shaking several times and directly analyzed using DART-MS. The presence of synthetic drug adulterants was confirmed through the accurate m/z values and MS/MS data obtained via quadruple time of flight mass spectrometry (QTOF MS). Parameters for the DART source were systematically optimized, and the limits of detection (LODs) in herbal supplement matrices were measured. This method was successfully applied to examine five commercial herbal dietary supplements, and two of them proved to be adulterated with metformin without labeling.
草药补充剂中掺入合成药物是非法的。本研究开发了一种快速可靠的方法，即利用实时直接分析质谱（DART-MS）来鉴定作为草药膳食补充剂掺假剂的七种合成抗糖尿病药物。通过手动摇晃数次，用甲醇/水简单地从补充剂样品中提取，并直接使用 DART-MS 进行分析。通过四极飞行时间质谱（QTOF MS）获得的准确 m/z 值和 MS/MS 数据，确认了合成药物掺假剂的存在。系统地优化了 DART 源的参数，并测量了草药补充剂基质中的检测限（LOD）。该方法成功地应用于检测五种商业草药膳食补充剂，其中两种被证明未经标记就掺有二甲双胍。
