Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research, Ministry of Education, Hunan Normal University, Changsha 410081, China.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2010 Jul;27(7):893-902. doi: 10.1080/19440040903426710.
A sensitive and specific liquid chromatography-electrospray ionization mass spectrometry (LC/ESI-MS) method was developed for the analysis of 18 drugs used for the treatment of anti-hypertension, including diuretics, calcium antagonists, and angiogenesis-converting enzyme inhibitors (ACEI) as adulterants in dietary supplements and traditional Chinese medicines. Separation was accomplished on a Xtimate C18 reversed-phase column using a mixture of methanol, acetonitrile and 20 mM ammonium formate buffer (pH 3.2) as mobile phase. The method demonstrated linearity from 0.03 to 21.52 mg kg(-1). Limits of detection ranged from 6.5 to 86.0 microg kg(-1). The recoveries of spiked samples ranged from 71% to 109%. The procedure was successfully applied in routine inspection analysis.
建立了一种灵敏、专属的液质联用（LC/ESI-MS）方法，用于分析 18 种治疗高血压的药物，包括利尿剂、钙拮抗剂和血管紧张素转化酶抑制剂（ACEI），这些药物作为膳食补充剂和中药中的掺杂物。采用甲醇、乙腈和 20mM 甲酸铵缓冲液（pH3.2）的混合溶液作为流动相，在 Xtimate C18 反相柱上实现了分离。该方法的线性范围为 0.03 至 21.52mgkg(-1)。检测限范围为 6.5 至 86.0μgkg(-1)。加标样品的回收率在 71%至 109%之间。该程序成功应用于常规检验分析。
