Grunina A S, Rekubratskiĭ A V, Tsvetkova L I, Barmintseva A E, Vasil'eva E D, Kovalev K V, Poluéktova O G
Ontogenez. 2011 Mar-Apr;42(2):133-45.
Dispermic androgenesis was used to produce, for the first time, an androgenetic progeny of the Siberian sturgeon (Acipenser baerii) and the androgenetic nuclear cytoplasmic hybrids (Siberian sturgeon, A. baerii x Russian sturgeon, A. gueldenstaedtii) using cryopreserved sperm. Microsatellite DNA analysis confirmed exclusively paternal inheritance in the androgenetic progeny of Siberian sturgeon. Heterozygotes for certain microsatellite loci were detected among the androgenetic hybrids, thereby confirming a dispermic nature of androgenesis. According to the data of comparative morphological analysis, the obtained androgenetic hybrid, by the age of 15 months old, was completely identical to the paternal species. Both a female and a male were detected in the androgenetic sturgeon progenies, which is of interest for producing bisexual progenies via androgenesis. The data of this study confirm the feasibility of dispermic androgenesis using cryopreserved sperm to preserve and recover the gene pools of endangered sturgeon species.
首次利用双精入卵雄核发育，使用冷冻保存的精子培育出了西伯利亚鲟（Acipenser baerii）的雄核发育后代以及雄核发育核质杂种（西伯利亚鲟，A. baerii×俄罗斯鲟，A. gueldenstaedtii）。微卫星DNA分析证实，西伯利亚鲟雄核发育后代完全为父系遗传。在雄核发育杂种中检测到某些微卫星位点的杂合子，从而证实了雄核发育的双精入卵性质。根据比较形态学分析数据，所获得的雄核发育杂种在15月龄时与父本物种完全相同。在雄核发育的鲟鱼后代中检测到了雌性和雄性，这对于通过雄核发育产生双性后代具有重要意义。本研究数据证实了利用冷冻保存的精子进行双精入卵雄核发育以保护和恢复濒危鲟鱼物种基因库的可行性。
