RNA 与 DNA（NucliSENS EasyQ HIV-1 v1.2 与 Amplicor HIV-1 DNA test v1.5）在塞内加尔用于早期诊断婴儿 HIV-1 感染。
RNA versus DNA (NucliSENS EasyQ HIV-1 v1.2 versus Amplicor HIV-1 DNA test v1.5) for early diagnosis of HIV-1 infection in infants in Senegal.
机构信息
Laboratoire de Bactériologie Virologie, Hôpital Aristide le Dantec, Université Cheikh Anta Diop, Dakar, Senegal.
出版信息
J Clin Microbiol. 2011 Jul;49(7):2590-3. doi: 10.1128/JCM.02402-10. Epub 2011 May 4.
Abstract
The objective of this study was to compare the performance of the NucliSENS EasyQ HIV-1 v1.2 platform (bioMérieux, France) to the Amplicor HIV-1 DNA test v1.5 (Roche Molecular Systems, Switzerland) in detecting HIV-1 infection in infants using venipuncture-derived whole blood in tubes and dried blood spots. A total of 149 dried blood spots and 43 EDTA-anticoagulated peripheral blood samples were collected throughout Dakar and other areas in Senegal from infants and children aged 3 weeks to 24 months who were born to HIV-1-infected mothers. Samples were tested using the NucliSENS and Amplicor technologies. The NucliSENS and Amplicor results were 100% concordant using either EDTA-anticoagulated peripheral blood or dried blood spots. Compared to Amplicor, the sensitivity and specificity of the NucliSENS test were 100%. The NucliSENS EasyQ HIV-1 RNA assay performed as well as the Amplicor HIV-1 DNA test in detecting HIV-1 infection in infants. In addition, this platform can give an indication of the viral load baseline. The NucliSENS EasyQ platform is a good alternative for early infant diagnosis of HIV-1 infection.
摘要
本研究旨在比较 NucliSENS EasyQ HIV-1 v1.2 平台（法国生物梅里埃公司）与 Amplicor HIV-1 DNA 测试 v1.5（罗氏分子系统公司，瑞士）在使用静脉穿刺采集的全血管和干血斑检测来自感染 HIV-1 的母亲所生的 3 周至 24 个月龄婴儿中的 HIV-1 感染的性能。在达喀尔和塞内加尔的其他地区共采集了 149 个干血斑和 43 个 EDTA 抗凝外周血样本。使用 NucliSENS 和 Amplicor 技术对样本进行了检测。使用 EDTA 抗凝外周血或干血斑时，NucliSENS 和 Amplicor 的结果完全一致。与 Amplicor 相比，NucliSENS 检测的敏感性和特异性均为 100%。NucliSENS EasyQ HIV-1 RNA 检测法与 Amplicor HIV-1 DNA 检测法一样能够检测婴儿的 HIV-1 感染。此外，该平台还可以提供病毒载量基线的指示。NucliSENS EasyQ 平台是早期婴儿 HIV-1 感染诊断的良好替代方法。
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