The Jiangsu Province Key Laboratory of Neuroregeneration, Nantong University, Nantong 226001, People's Republic of China.
J Mol Histol. 2011 Jun;42(3):265-71. doi: 10.1007/s10735-011-9330-x. Epub 2011 May 5.
The protein TFIIB is a general transcription initiation factor that plays a pivotal role in the preinitiation complex (PIC) and selects the transcription initiation site. However, its distribution and function in the central nervous system (CNS) remains unclear. In the present study, we mainly investigated the expression and cellular localization of TFIIB during traumatic brain injury (TBI). Western blot analysis revealed that TFIIB was present in normal rat brain cortex. It gradually increased, reached a peak at the 5th day after TBI, and then decreased. Importantly, more TFIIB was colocalized with astrocytes and microglia, which are largely proliferated. In addition, Western blot detection showed that the 5th day post injury was also the proliferation peak indicated by the elevated expression of PCNA. Importantly, injury-induced expression of TFIIB was colabelled by proliferating cell nuclear antigen (proliferating cells marker). These data suggested that TFIIB may be implicated in the proliferation of astrocytes and microglia and the recovery of neurological outcomes. But the inherent mechanisms remained unknown. Further studies are needed to confirm the exact role of TFIIB after brain injury.
TFIIB 蛋白是一种通用转录起始因子，在起始前复合物（PIC）中发挥关键作用，并选择转录起始位点。然而，它在中枢神经系统（CNS）中的分布和功能尚不清楚。在本研究中，我们主要研究了 TFIIB 在创伤性脑损伤（TBI）中的表达和细胞定位。Western blot 分析显示 TFIIB 存在于正常大鼠大脑皮质中。它逐渐增加，在 TBI 后第 5 天达到峰值，然后下降。重要的是，更多的 TFIIB 与大量增殖的星形胶质细胞和小胶质细胞共定位。此外，Western blot 检测显示，损伤后第 5 天也是增殖细胞核抗原（增殖细胞标志物）表达升高所表明的增殖高峰。重要的是，损伤诱导的 TFIIB 表达与增殖细胞核抗原（增殖细胞标志物）共标记。这些数据表明，TFIIB 可能参与星形胶质细胞和小胶质细胞的增殖以及神经功能恢复。但内在机制尚不清楚。需要进一步研究来确认脑损伤后 TFIIB 的确切作用。
