Rai Vikrant, Mendoza-Mari Yssel, Radwan Mohamed M, Brazdzionis James, Connett David A, Miulli Dan E, Agrawal Devendra K
Department of Translational Research, College of Osteopathic Medicine of the Pacific, Western University of Health Sciences, Pomona CA 91766, USA.
J Bioinform Syst Biol. 2024;7(1):81-91. doi: 10.26502/jbsb.5107080. Epub 2024 Mar 5.
Traumatic brain injury (TBI) is a leading cause of morbidity, disability, and mortality worldwide. Motor and cognitive deficits and emotional disturbances are long-term consequences of TBI. A lack of effective treatment for TBI-induced neural damage, functional impairments, and cognitive deficits makes it challenging in the recovery following TBI. One of the reasons may be the lack of knowledge underlying the complex pathophysiology of TBI and the regulatory factors involved in the cellular and molecular mechanisms of inflammation, neural regeneration, and injury repair. These mechanisms involve a change in the expression of various proteins encoded by genes whose expression is regulated by transcription factors (TFs) at the transcriptional level and microRNA (miRs) at the mRNA level. In this pilot study, we performed the RNA sequencing of injured tissues and non-injured tissues from the brain of Yucatan miniswine and analyzed the sequencing data for differentially expressed genes (DEGs) and the TFs and miRs regulating the expression of DEGs using in-silico analysis. We also compared the effect of the electromagnetic field (EMF) applied to the injured miniswine on the expression profile of various DEGs. The results of this pilot study revealed a few DEGs that were significantly upregulated in the injured brain tissue and the EMF stimulation showed effect on their expression profile.
创伤性脑损伤（TBI）是全球发病、致残和致死的主要原因之一。运动和认知缺陷以及情绪障碍是TBI的长期后果。缺乏针对TBI所致神经损伤、功能障碍和认知缺陷的有效治疗方法，使得TBI后的恢复具有挑战性。原因之一可能是缺乏对TBI复杂病理生理学以及炎症、神经再生和损伤修复的细胞与分子机制中涉及的调节因子的了解。这些机制涉及由基因编码的各种蛋白质表达的变化，其表达在转录水平上受转录因子（TFs）调控，在mRNA水平上受微小RNA（miRs）调控。在这项初步研究中，我们对尤卡坦小型猪大脑的损伤组织和未损伤组织进行了RNA测序，并使用计算机分析对差异表达基因（DEGs）的测序数据以及调控DEGs表达的TFs和miRs进行了分析。我们还比较了施加于受伤小型猪的电磁场（EMF）对各种DEGs表达谱的影响。这项初步研究的结果揭示了一些在受伤脑组织中显著上调的DEGs，并且EMF刺激对它们的表达谱有影响。
