Department of Immunology, Medical College, Nantong University, 19 Qi-Xiu Road, Nantong, Jiangsu Province, 226001, People's Republic of China.
J Mol Neurosci. 2013 Mar;49(3):491-8. doi: 10.1007/s12031-012-9865-7. Epub 2012 Aug 7.
Transcription Initiation Factor IIB (TFIIB), as a general transcription factor, plays an essential role in preinitiation complex assembly and transcription initiation by recruiting RNA polymerase II to the promoter. However, its distribution and function in peripheral system lesion and repair were still unknown. Here, we investigated the spatiotemporal expression of TFIIB in an acute sciatic nerve crush model in adult rats. Western blot analysis revealed that TFIIB was expressed in normal sciatic nerve. It gradually increased, reached a peak at the seventh day after crush, and then returned to the normal level at 4 weeks. We observed that TFIIB expressed mainly increased in Schwann cells and co-localized with Oct-6. In vitro, we induced Schwann cell differentiation with cyclic adenosine monophosphate (cAMP) and found that TFIIB expression was increased in the differentiated process. TFIIB-specific siRNA inhibited cAMP-induced Schwann cell morphological change and the expression of P0. Collectively, we hypothesized peripheral nerve crush-induced upregulation of TFIIB in the sciatic nerve was associated with Schwann cell differentiation.
转录起始因子 IIB（TFIIB）作为一种通用转录因子，通过招募 RNA 聚合酶 II 到启动子，在起始复合物组装和转录起始中发挥重要作用。然而，其在外周系统损伤和修复中的分布和功能仍不清楚。在这里，我们研究了 TFIIB 在成年大鼠急性坐骨神经挤压模型中的时空表达。Western blot 分析显示 TFIIB 在正常坐骨神经中表达。它逐渐增加，在挤压后第 7 天达到峰值，然后在 4 周时恢复到正常水平。我们观察到 TFIIB 主要在施万细胞中表达，并与 Oct-6 共定位。在体外，我们用环磷酸腺苷（cAMP）诱导施万细胞分化，发现 TFIIB 的表达在分化过程中增加。TFIIB 特异性 siRNA 抑制 cAMP 诱导的施万细胞形态变化和 P0 的表达。总的来说，我们假设坐骨神经挤压诱导的 TFIIB 上调与施万细胞分化有关。
