Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, People's Republic of China.
J Mol Neurosci. 2013 Feb;49(2):387-94. doi: 10.1007/s12031-012-9901-7. Epub 2012 Oct 24.
The KIF3 subunit KIF3B was proved to be associated with mitosis. It has been known to be engaged in intracellular transport of neurons. To elucidate the certain expression and biological function in central nervous system, we performed an acute spinal cord contusion injury model in adult rats. Western blot analysis indicated a marked upregulation of KIF3B after spinal cord injury (SCI). Immunohistochemistry revealed wide distribution of KIF3B in spinal cord, including neurons and glial cells. Double immunofluorescent staining for proliferating cell nuclear antigen and phenotype-specific markers showed increases of KIF3B expression in proliferating microglia and astrocytes. Our data suggest that KIF3B may be implicated in the proliferation of microglia and astrocytes after SCI.
KIF3 亚基 KIF3B 已被证明与有丝分裂有关。它被认为参与神经元的细胞内运输。为了阐明中枢神经系统中的特定表达和生物学功能，我们在成年大鼠中建立了急性脊髓挫伤损伤模型。Western blot 分析表明，脊髓损伤 (SCI) 后 KIF3B 的表达明显上调。免疫组织化学显示 KIF3B 在脊髓中的广泛分布，包括神经元和神经胶质细胞。增殖细胞核抗原和表型特异性标志物的双重免疫荧光染色显示，增殖的小胶质细胞和星形胶质细胞中 KIF3B 的表达增加。我们的数据表明，KIF3B 可能参与 SCI 后小胶质细胞和星形胶质细胞的增殖。
