用 N-乙基马来酰亚胺稳定全血后检测谷胱甘肽。
Detection of glutathione in whole blood after stabilization with N-ethylmaleimide.
机构信息
Department of Evolutionary Biology, Laboratory of Pharmacology and Toxicology, University of Siena, 53100 Siena, Italy.
出版信息
Anal Biochem. 2011 Aug 1;415(1):81-3. doi: 10.1016/j.ab.2011.04.013. Epub 2011 Apr 20.
The measurement of glutathione is a challenging task in that, during sample manipulation, a large percentage of this compound can be artificially oxidized. Here a high-performance liquid chromatography (HPLC) method to measure reduced glutathione in blood, based on the analysis of its conjugate with N-ethylmaleimide, has been developed and validated. The method is linear in the concentration range 0.1-2 mM, and the lower limit of quantification is 0.05 mM. Blood samples treated with N-ethylmaleimide are stable at -20°C for 90 days. This method has simplicity and rapidity as its main advantages; therefore, it is particularly suitable for large-scale clinical studies.
谷胱甘肽的测量是一项具有挑战性的任务，因为在样品处理过程中，这种化合物的很大一部分可以被人为氧化。在这里，开发并验证了一种基于其与 N-乙基马来酰亚胺的缀合物分析的高效液相色谱 (HPLC) 方法来测量血液中的还原型谷胱甘肽。该方法在 0.1-2mM 的浓度范围内呈线性，定量下限为 0.05mM。用 N-乙基马来酰亚胺处理的血液样品在-20°C 下稳定 90 天。该方法具有简单和快速的主要优点；因此，它特别适合于大规模的临床研究。
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