Yei S P, Chowdhury S I, Bhat B M, Conley A J, Wold W S, Batterson W
Department of Microbiology, University of Texas Dental Branch, University of Texas Health Science Center, Houston 77030.
J Virol. 1990 Mar;64(3):1124-34. doi: 10.1128/JVI.64.3.1124-1134.1990.
We describe the characterization of the herpes simplex virus type 2 (HSV-2) gene encoding infected cell protein 32 (ICP32) and virion protein 19c (VP19c). We also demonstrate that the HSV-1 UL38/ORF.553 open reading frame (ORF), which has been shown to specify a viral protein essential for capsid formation (B. Pertuiset, M. Boccara, J. Cebrian, N. Berthelot, S. Chousterman, F. Puvian-Dutilleul, J. Sisman, and P. Sheldrick, J. Virol. 63: 2169-2179, 1989), must encode the cognate HSV type 1 (HSV-1) ICP32/VP19c protein. The region of the HSV-2 genome deduced to contain the gene specifying ICP32/VP19c was isolated and subcloned, and the nucleotide sequence of 2,158 base pairs of HSV-2 DNA mapping immediately upstream of the gene encoding the large subunit of the viral ribonucleotide reductase was determined. This region of the HSV-2 genome contains a large ORF capable of encoding two related 50,538- and 49,472-molecular-weight polypeptides. Direct evidence that this ORF encodes HSV-2 ICP32/VP19c was provided by immunoblotting experiments that utilized antisera directed against synthetic oligopeptides corresponding to internal portions of the predicted polypeptides encoded by the HSV-2 ORF or antisera directed against a TrpE/HSV-2 ORF fusion protein. The type-common immunoreactivity of the two antisera and comparison of the primary amino acid sequences of the predicted products of the HSV-2 ORF and the equivalent genomic region of HSV-1 provided evidence that the HSV-1 UL38 ORF encodes the HSV-1 ICP32/VP19c. Analysis of the expression of the HSV-1 and HSV-2 ICP32/VP19c cognate proteins indicated that there may be differences in their modes of synthesis. Comparison of the predicted structure of the HSV-2 ICP32/VP19c protein with the structures of related proteins encoded by other herpes viruses suggested that the internal capsid architecture of the herpes family of viruses varies substantially.
我们描述了编码感染细胞蛋白32（ICP32）和病毒体蛋白19c（VP19c）的2型单纯疱疹病毒（HSV - 2）基因的特征。我们还证明，已显示指定衣壳形成所必需的病毒蛋白的HSV - 1 UL38 / ORF.553开放阅读框（ORF）（B. Pertuiset、M. Boccara、J. Cebrian、N. Berthelot、S. Chousterman、F. Puvian - Dutilleul、J. Sisman和P. Sheldrick，《病毒学杂志》63: 2169 - 2179，1989）必定编码同源的1型单纯疱疹病毒（HSV - 1）ICP32 / VP19c蛋白。推导认为包含指定ICP32 / VP19c基因的HSV - 2基因组区域被分离并亚克隆，并且测定了紧邻病毒核糖核苷酸还原酶大亚基编码基因上游的2158个碱基对的HSV - 2 DNA的核苷酸序列。HSV - 2基因组的该区域包含一个能够编码两种相关的分子量分别为50,538和49,472的多肽的大ORF。利用针对与HSV - 2 ORF编码的预测多肽内部部分相对应的合成寡肽的抗血清或针对TrpE / HSV - 2 ORF融合蛋白的抗血清进行的免疫印迹实验，提供了该ORF编码HSV - 2 ICP32 / VP19c的直接证据。两种抗血清的型共同免疫反应性以及HSV - 2 ORF预测产物与HSV - 1等效基因组区域的一级氨基酸序列比较，提供了HSV - 1 UL38 ORF编码HSV - 1 ICP32 / VP19c的证据。对HSV - 1和HSV - 2 ICP32 / VP19c同源蛋白表达的分析表明，它们的合成模式可能存在差异。将HSV - 2 ICP32 / VP19c蛋白的预测结构与其他疱疹病毒编码的相关蛋白的结构进行比较，表明疱疹病毒家族的内部衣壳结构有很大差异。
