Department of Biotechnology, Interdisciplinary Program of Graduate School for Bioenergy and Biomaterials, Bioenergy Research Center, Chonnam National University, South Korea.
Plant Signal Behav. 2011 Jun;6(6):881-3. doi: 10.4161/psb.6.6.15280. Epub 2011 Jun 1.
Rice (Oryza sativa cv. Dongjin) plants responded to treatment with methanol by inducing the synthesis of secondary metabolites such as serotonin derivatives which include feruloylserotonin and 4-coumaroylserotonin. This response was not only a dose dependence on methanol showing a maximum effect with 1% methanol concentration, but also methanol specific. No other solvents such as ethanol, atetaldehyde, isopropanol, formaldehyde, and formic acid showed the induced synthesis of serotonin derivatives as methanol did. The methanol induced synthesis of serotonin derivatives was completely blocked by the addition of abscisic acid (ABA), and significantly inhibited by the additions of zeatin and indoleacetic acid (IAA). However, gibberellic acid (GA) had little effect on the action of methanol. Finally, the induced synthesis of serotonin derivatives upon methanol treatment was closely associated with the transient increase in the activity of key enzyme of serotonin N-hydroxycinnamoyl transferase (SHT) which catalyzes the condensation of serotonin and phenolic-CoA into serotonin derivatives.
水稻（Oryza sativa cv. Dongjin）植株对甲醇处理的反应是诱导合成次生代谢物，如 5-羟色胺衍生物，包括阿魏酰 5-羟色胺和 4-香豆酰 5-羟色胺。这种反应不仅对甲醇有剂量依赖性，在 1%甲醇浓度下表现出最大效应，而且是甲醇特异性的。没有其他溶剂，如乙醇、乙醛、异丙醇、甲醛和甲酸，像甲醇那样诱导 5-羟色胺衍生物的合成。添加脱落酸（ABA）完全阻断了甲醇诱导的 5-羟色胺衍生物的合成，而添加玉米素和吲哚乙酸（IAA）则显著抑制了 5-羟色胺衍生物的合成。然而，赤霉素（GA）对甲醇的作用影响不大。最后，甲醇处理诱导的 5-羟色胺衍生物的合成与 5-羟色胺 N-羟基肉桂酰转移酶（SHT）关键酶活性的短暂增加密切相关，该酶催化 5-羟色胺和酚-CoA 缩合生成 5-羟色胺衍生物。
